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Literature summary extracted from

  • Schulte, A.E.; Van der Heijden, R.; Verpoorte, R.
    Purification and characterization of phosphomevalonate kinase from Catharanthus roseus (1999), Phytochemistry, 52, 975-983.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.4.2 sulfhydryl reagents are not essential to protect the activity of phosphomevalonate kinase Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.2 0.22
-
 ATP pH 7.5, 30°C Catharanthus roseus
2.7.4.2 0.35
-
 phosphomevalonate pH 7.5, 30°C Catharanthus roseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.2 Mg2+ divalent cation required for activity, Mg2+ most effective Catharanthus roseus
2.7.4.2 Mn2+ 52% of activity with Mg2+ Catharanthus roseus
2.7.4.2 Ni2+ 4.7% of activity with Mg2+ Catharanthus roseus
2.7.4.2 Zn2+ 22% of activity with Mg2+ Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.2 128000
-
 gel filtration Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate Catharanthus roseus involved in isoprenoid biosynthesis pathway ADP + 5-diphosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.2 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.2 Q Sepharose, Phenyl Sepharose, Sephacryl S200, Mono Q, gel filtration, Shodex KW803 Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.2 cell suspension culture
-
 Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.2 0.21
-
-
 Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.2 ATP + (R)-5-phosphomevalonate involved in isoprenoid biosynthesis pathway Catharanthus roseus ADP + 5-diphosphomevalonate
-
 ?
2.7.4.2 ATP + 5-phosphomevalonate
-
 Catharanthus roseus ADP + 5-diphosphomevalonate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.2 7 9 sharp drop in activity below pH 7.0 and above pH 9.0 Catharanthus roseus