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Literature summary extracted from

  • Kohno, H.; Kumagai, H.; Tochikura, T.
    Purification and properties of pyrimidine nucleoside monophosphate kinase from baker‘s yeast (1983), Agric. Biol. Chem., 47, 19-24.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.14 dithiothreitol activates Saccharomyces cerevisiae
2.7.4.14 dithiothreitol 1 mM, activates Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.14 CMP competitive inhibition of UMP phosphorylation Saccharomyces cerevisiae
2.7.4.14 dCMP competitive inhibition of UMP phosphorylation Saccharomyces cerevisiae
2.7.4.14 HgCl2 0.1 mM, 88% inhibition Saccharomyces cerevisiae
2.7.4.14 NEM 1.0 mM; 41% inhibition Saccharomyces cerevisiae
2.7.4.14 PCMB 0.1 mM, 69% loss of activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.14 0.052
-
 UMP pH 7.5, 25°C Saccharomyces cerevisiae
2.7.4.14 0.071
-
 CMP pH 7.5, 25°C Saccharomyces cerevisiae
2.7.4.14 0.54
-
 dCMP pH 7.5, 25°C Saccharomyces cerevisiae
2.7.4.14 8.5
-
 dUMP pH 7.5, 25°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.14 Co2+ divalent cation required, Mn2+ can substitute for Mg2+ Saccharomyces cerevisiae
2.7.4.14 Mg2+ divalent cation required, Mg2+ is most effective Saccharomyces cerevisiae
2.7.4.14 Mn2+ divalent cation required, Mn2+ can substitute for Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.14 26000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.14
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.14 11.1
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + CMP
-
 Saccharomyces cerevisiae ADP + CDP
-
 ?
2.7.4.14 ATP + dCMP
-
 Saccharomyces cerevisiae ADP + dCDP
-
 ?
2.7.4.14 ATP + dUMP
-
 Saccharomyces cerevisiae ADP + dUDP
-
 ?
2.7.4.14 ATP + UMP
-
 Saccharomyces cerevisiae ADP + UDP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.14 4
-
 at pH 7 the enzyme is most stable when kept at 4°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.14 7.5
-
 reaction with UMP and ATP Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.14 4 9 pH 4.0: about 40% of maximal activity, pH 9.0: about 65% of maximal activity, reaction with UMP and ATP Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.14 7
-
 at pH 7.0, the enzyme is most stable when kept at 4°C Saccharomyces cerevisiae