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Literature summary extracted from

  • Mullan, A.; Quinn, J.P.; McGrath, J.W.
    A nonradioactive method for the assay of polyphosphate kinase activity and its application in the study of polyphosphate metabolism in Burkholderia cepacia (2002), Anal. Biochem., 308, 294-299.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 ADP 0.025 mM, 0.05 mM, 0.1 mM and 0.25 mM, 56%, 68%, 77% and 100% inhibition, respectively of polyphosphate synthesis in crude extracts Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 0.83
-
 ATP pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, detrmined with Robinson and Wood method Burkholderia cepacia
2.7.4.1 1.18
-
 ATP pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, kinetic analysis Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ maximal activation at 10 mM Burkholderia cepacia
2.7.4.1 Mn2+ can replace Mg2+ to some extent Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Burkholderia cepacia
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.1 0.13
-
 polyphosphate kinase activity in crude extracts measured with a modified toluidine blue assay Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n no activity with CTP, GTP and TTP Burkholderia cepacia ADP + (phosphate)n+1
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.1 37
-
-
 Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 7
-
-
 Burkholderia cepacia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.1 5.5 8 approx. 10% of maximal activity at pH 5.5 and pH 8.0 respectively Burkholderia cepacia