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Literature summary extracted from

  • Jergil, B.; Dixon, G.H.
    Protamine kinase from rainbow trout testis. Partial purification and characterization (1970), J. Biol. Chem., 245, 425-434.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.1 2-mercaptoethanol activation, can replace DTT Oncorhynchus mykiss
2.7.11.1 3',5'-AMP
-
 Oncorhynchus mykiss

General Stability

EC Number General Stability Organism
2.7.11.1 freeze-thawing inactivates Oncorhynchus mykiss

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Co2+ not Oncorhynchus mykiss
2.7.11.1 high ionic strength activation, e.g. NaCl, NH4Cl, KCl, sodium acetate, essential for maximal phosphate incorporation with protamine as substrate, histone phosphorylation is suppressed Oncorhynchus mykiss
2.7.11.1 Mg2+ requirement Oncorhynchus mykiss
2.7.11.1 Mn2+ can replace Mg2+ with 8% efficiency Oncorhynchus mykiss
2.7.11.1 additional information no activation by Ca2+ or Zn2+ Oncorhynchus mykiss

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Oncorhynchus mykiss
-
 rainbow trout
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 partial Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.1 testis
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.1 0.07
-
-
 Oncorhynchus mykiss

Storage Stability

EC Number Storage Stability Organism
2.7.11.1 0-4°C, partially purified preparation, several weeks Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + casein poor substrate Oncorhynchus mykiss ?
-
 ?
2.7.11.1 ATP + histone fraction II slightly lysine-rich, from trout testes Oncorhynchus mykiss ?
-
 ?
2.7.11.1 ATP + protamine preferred substrate Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?
2.7.11.1 ATP + protamine transfers terminal phosphoryl group from ATP into O-phosphoseryl linkages in the acceptor molecule Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?
2.7.11.1 ATP + protamine no substrates are GTP Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?
2.7.11.1 ATP + protamine no substrates are GTP or UTP Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?
2.7.11.1 ATP + protamine phosphorylates histone fraction I (lysine-rich) and histone fractions III and IV (arginine-rich), from trout testes Oncorhynchus mykiss ADP + O-phosphoprotamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 20
-
 assay at Oncorhynchus mykiss

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 7.8 8 at high salt concentration Oncorhynchus mykiss

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.1 6.4 8.6 about half-maximal activity at pH 6.4 and about 65% of maximal activity at pH 8.6, at high salt concentration Oncorhynchus mykiss