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Literature summary extracted from

  • Murga, C.; Ruiz-Gomez, A.; Garcia-Higuera, I.; Kim, C.M.; Benovic, J.L.; Mayor, F.Jr.
    High affinity binding of beta-adrenergic receptor kinase to microsomal membranes. Modulation of the activity of bound kinase by heterotrimeric G protein activation (1996), J. Biol. Chem., 271, 985-994.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 AlF4- enhances beta-ARK activity upon stimulation of heterotrimeric G proteins Bos taurus
2.7.11.15 G protein betagamma-subunit activates, binding domain is localized to the C-terminal region of beta-ARK Bos taurus
2.7.11.15 mastoparan/guanosine 5'-(3-O-thio)triphosphate enhances beta-ARK activity upon stimulation of heterotrimeric G proteins Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system Bos taurus
2.7.11.15 cloning of GST-beta-ARK fusion proteins and expression in Escherichia coli AG1 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.15 heparin
-
 Bos taurus
2.7.11.15 additional information marked inhibition of rhodopsin or synthetic peptide phosphorylation in the presence of increasing amounts of microsomal membranes, bound enzyme is less able to interact with its substrate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.15 microsome associates with intracellular microsomal membranes, mechanism, modulation of binding of enzyme, main determinants of binding appear to be localized to an 60 amino acid residue stretch, residues 88 to 145, G protein betagamma subunits are not the main anchor in the membranes Bos taurus
-
-
2.7.11.15 additional information complex modulation of the subcellular distribution of beta-ARK Bos taurus
-
-
2.7.11.15 plasma membrane soluble enzyme, that transiently translocates to the plasma membrane Bos taurus 5886
-
2.7.11.15 soluble soluble enzyme, that transiently translocates to the plasma membrane Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.15 Mg2+ increases binding of beta-ARK to microsomal membranes Bos taurus
2.7.11.15 Mn2+ Mn2+ can partially substitute Mg2+ in increasing the binding of beta-ARK to microsomal membranes Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus desensitization of beta-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus agonist-occupied form of the receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 additional information Bos taurus beta-ARK activity is regulated by endogenous G proteins in different intracellular locations ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Bos taurus
-
 beta-ARK 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 recombinant beta-ARK 1 expressed in Sf9 cells Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.15 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor specifically phosphorylates the agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor desensitization of beta-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + rhodopsin light-dependent, actual substrate: light-bleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + RRREEEEESAAA
-
 Bos taurus ?
-
 ?
2.7.11.15 additional information beta-ARK interacts rapidly with a high affinity binding site present in salt-stripped rat liver microsomal membranes, modulation of binding of enzyme Bos taurus ?
-
 ?
2.7.11.15 additional information phosphorylates G protein coupled receptors in an agonist-dependent manner Bos taurus ?
-
 ?
2.7.11.15 additional information beta-ARK activity is regulated by endogenous G proteins in different intracellular locations Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 7.5
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Bos taurus