Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Haske, T.N.; DeBlasi, A.; LeVine, H.
    An intact N terminus of the gamma subunit is required for the Gbetagamma stimulation of rhodopsin phosphorylation by human beta-adrenergic receptor kinase-1 but not for kinase binding (1996), J. Biol. Chem., 271, 2941-2948.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 G protein betagamma-subunit from brain, stimulates the phosphorylation of rhodopsin, but not of the peptide RRREEEEESAAA, an intact N-terminus of the gamma subunit is required for stimulation, but not for kinase binding, endoprotease Lys-C blocks stimulation, Gbetagamma binds to the C-terminal region of beta-ARK containing the pleckstrin homology domain, role of the G protein gamma-subunit Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 DNAs encoding the C-terminal domains Gly556-Ser670 and Pro466-Leu689 are cloned and expressed in Escherichia coli Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Homo sapiens
-
 recombinant beta-ARK 1 holoenzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 recombinant beta-ARK 1, expressed in Sf9 cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor
-
 Homo sapiens ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Homo sapiens ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + RRREEEEESAAA
-
 Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Homo sapiens