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Literature summary extracted from

  • Söhlemann, P.; Hekman, M.; Buchen, C.; Elce, J.S.; Lohse, M.J.
    Purification and functional characterization of beta-adrenergic receptor kinase expressed in insect cells (1993), FEBS Lett., 324, 59-62.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system Homo sapiens
2.7.11.15 beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.15 0.0038
-
 rhodopsin pH 7.5, 30°C, recombinant beta-ARK 1 Bos taurus
2.7.11.15 0.022
-
 ATP pH 7.5, 30°C, recombinant beta-ARK 1, rhodopsin as substrate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.15 cytosol
-
 Homo sapiens 5829
-
2.7.11.15 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.15 Mg2+ requirement Homo sapiens
2.7.11.15 Mg2+ requirement Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor Homo sapiens natural substrate: beta2-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus natural substrate: beta2-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Homo sapiens agonist-occupied form of the receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus agonist-occupied form of the receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Homo sapiens involved in homologous desensitization of beta-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus involved in homologous desensitization of beta-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Bos taurus
-
 enzyme exists in 2 subtypes: beta-ARK 1 and beta-ARK 2
-
2.7.11.15 Bos taurus
-
 major isoform beta-ARK 1
-
2.7.11.15 Homo sapiens
-
 major isoform beta-ARK 1
-
2.7.11.15 Homo sapiens
-
 enzyme exists in 2 subtypes: beta-ARK1 and 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 recombinant beta-ARK 1 expressed in Sf9 cells Bos taurus
2.7.11.15 recombinant beta-ARK 1, expressed in Sf9 cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.15 brain
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor specifically phosphorylates the agonist-occupied form of the receptor Homo sapiens ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor specifically phosphorylates the agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor human beta2-adrenergic receptor Homo sapiens ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor human beta2-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor incorporation of up to 5 mol phosphate/mol receptor Homo sapiens ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor incorporation of up to 5 mol phosphate/mol receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta-AR from Sf9 cells Homo sapiens ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta-AR from Sf9 cells Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor natural substrate: beta2-adrenergic receptor Homo sapiens ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor natural substrate: beta2-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor agonist-occupied form of the receptor Homo sapiens ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor involved in homologous desensitization of beta-adrenergic receptor Homo sapiens ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor involved in homologous desensitization of beta-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Homo sapiens ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Bos taurus ADP + phosphorhodopsin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Homo sapiens
2.7.11.15 30
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Bos taurus
2.7.11.15 ATP cofactor ATP Homo sapiens