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Literature summary extracted from

  • Harwood, H.J.; Brandt, K.G.; Rodwell, V.W.
    Allosteric activation of rat liver cytosolic 3-hydroxy-3-methylglutaryl coenzyme A reductase kinase by nucleoside diphosphates (1984), J. Biol. Chem., 259, 2810-2815.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.31 ADP requirement Rattus norvegicus
2.7.11.31 alpha,beta-methylene-ADP allosteric activator, can replace ADP, with 66% efficiency with bovine serum albumin as substrate Rattus norvegicus
2.7.11.31 CDP allosteric activator Rattus norvegicus
2.7.11.31 additional information not activated by cAMP Rattus norvegicus
2.7.11.31 UDP allosteric activator Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.31 0.14
-
 ATP pH 7.5, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.31 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.31 56000
-
 x * 56000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] Rattus norvegicus inactivates EC 1.1.1.34 by phosphorylation ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.31 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.31
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.31 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.31 additional information
-
 3.130 nU/mg Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.11.31 -196°C, stored in liquid nitrogen, stable for several months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.31 ATP + bovine serum albumin fraction V Rattus norvegicus ADP + [bovine serum albumin] phosphate
-
 ?
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)]
-
 Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
2.7.11.31 ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] inactivates EC 1.1.1.34 by phosphorylation Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
2.7.11.31 dATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphorylation at about 90% the rate of ATP Rattus norvegicus dADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
2.7.11.31 GTP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphorylation at about 30% the rate of ATP Rattus norvegicus GDP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
2.7.11.31 ITP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphorylation at about 10% the rate of ATP Rattus norvegicus IDP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
2.7.11.31 UTP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphorylation at about 5% the rate of ATP Rattus norvegicus UDP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.31 ? x * 56000, SDS-PAGE Rattus norvegicus