EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.231 | AMP | stimulates phosphorolytic activity at 10 mM | Agaricus bisporus | |
2.4.1.231 | ATP | stimulates synthetic activity at 10 mM | Agaricus bisporus |
EC Number | General Stability | Organism |
---|---|---|
2.4.1.231 | highly unstable during purification, glycerol, phosphate, dithiothreitol and PMSF stabilize | Agaricus bisporus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.231 | 1-deoxynojirimycin | 88% inhibition at 10 mM | Agaricus bisporus | |
2.4.1.231 | ADP | 34% inhibition at 10 mM | Agaricus bisporus | |
2.4.1.231 | ATP | inhibits trehalose degradation, but stimulates synthetic activity | Agaricus bisporus | |
2.4.1.231 | CoCl2 | 39% inhibition at 1 mM | Agaricus bisporus | |
2.4.1.231 | CuSO4 | 37% inhibition at 1 mM | Agaricus bisporus | |
2.4.1.231 | phosphate | inhibits synthetic activity | Agaricus bisporus | |
2.4.1.231 | validamycin A | 99% inhibition at 10 mM | Agaricus bisporus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.231 | 4.7 | - |
phosphate | 30°C, pH 7 | Agaricus bisporus | |
2.4.1.231 | 6.3 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 7 | Agaricus bisporus | |
2.4.1.231 | 24 | - |
alpha-D-glucose | 30°C, pH 7 | Agaricus bisporus | |
2.4.1.231 | 61 | - |
alpha,alpha-trehalose | 30°C, pH 7 | Agaricus bisporus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.231 | 61000 | - |
4 * 61000, SDS-PAGE | Agaricus bisporus |
2.4.1.231 | 240000 | - |
native PAGE, gel filtration | Agaricus bisporus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.231 | alpha,alpha-trehalose + phosphate | Agaricus bisporus | key role in trehalose metabolism | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.231 | alpha,alpha-trehalose + phosphate | Agaricus bisporus Horst U1 | key role in trehalose metabolism | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.231 | Agaricus bisporus | - |
- |
- |
2.4.1.231 | Agaricus bisporus Horst U1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.231 | - |
Agaricus bisporus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.1.231 | alpha,alpha-trehalose + phosphate = alpha-D-glucose + alpha-D-glucose 1-phosphate | mechanism | Agaricus bisporus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.231 | fruit body | - |
Agaricus bisporus | - |
2.4.1.231 | mycelium | - |
Agaricus bisporus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.231 | 4.35 | - |
- |
Agaricus bisporus |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.231 | -80°C, stable for at least 6 months | Agaricus bisporus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.231 | alpha,alpha-trehalose + phosphate | - |
Agaricus bisporus | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.231 | alpha,alpha-trehalose + phosphate | key role in trehalose metabolism | Agaricus bisporus | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.231 | alpha,alpha-trehalose + phosphate | - |
Agaricus bisporus Horst U1 | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
2.4.1.231 | alpha,alpha-trehalose + phosphate | key role in trehalose metabolism | Agaricus bisporus Horst U1 | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.231 | tetramer | 4 * 61000, SDS-PAGE | Agaricus bisporus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.231 | 40 | - |
almost complete loss of activity after 60 min | Agaricus bisporus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.231 | 6 | 7 | synthesis of trehalose | Agaricus bisporus |
2.4.1.231 | 6 | 7.5 | phosphorolytic reaction | Agaricus bisporus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.231 | 2 | - |
phosphate | 30°C, pH 7 | Agaricus bisporus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.4.1.231 | Agaricus bisporus | isoelectric focusing between pH 3-9 and pH 3-10 | 4.8 | 4.7 |