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Literature summary extracted from

  • Groot Wassink, J.W.D.; Reed, D.W.; Kolenovsky, A.D.
    Immunopurification and immunocharacterization of the glucosinolate biosynthetic enzyme thiohydroximate S-glucosyltransferase (1994), Plant Physiol., 105, 425-433.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.195 agriculture glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways Brassica napus
2.4.1.195 agriculture glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways Brassica oleracea
2.4.1.195 nutrition glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways Brassica napus
2.4.1.195 nutrition glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.195 55500
-
-
 Brassica rapa subsp. oleifera
2.4.1.195 55500
-
-
 Brassica juncea
2.4.1.195 55500
-
 SDS-PAGE Brassica oleracea
2.4.1.195 57000
-
-
 Brassica nigra
2.4.1.195 57600
-
 gel filtration Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica napus
-
 UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica oleracea
-
 UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica rapa subsp. oleifera
-
 UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica juncea
-
 UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica nigra
-
 UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica carinata
-
 UDP + desulfoglucotropeolin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.195 Brassica carinata
-
-
-
2.4.1.195 Brassica juncea
-
-
-
2.4.1.195 Brassica napus
-
-
-
2.4.1.195 Brassica nigra
-
-
-
2.4.1.195 Brassica oleracea
-
 cauliflower, ssp. botrytis cv Snowball, self-blanching type white and open type brown
-
2.4.1.195 Brassica rapa subsp. oleifera
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.195 floret
-
 Brassica oleracea
-
2.4.1.195 leaf
-
 Brassica oleracea
-
2.4.1.195 seedling
-
 Brassica napus
-
2.4.1.195 seedling
-
 Brassica oleracea
-
2.4.1.195 seedling
-
 Brassica juncea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.195 3.2
-
-
 Brassica oleracea

Storage Stability

EC Number Storage Stability Organism
2.4.1.195 -20°, crude extract stored frozen without loss of activity for 1 year Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica napus UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica oleracea UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica rapa subsp. oleifera UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica juncea UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica nigra UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica carinata UDP + desulfoglucotropeolin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.195 monomer
-
 Brassica napus
2.4.1.195 monomer 1 * 55500, SDS-PAGE Brassica oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.195 Brassica oleracea isoenzymes with different pI values
-
 4.8
2.4.1.195 Brassica oleracea isoenzymes with different pI values
-
 4.95