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Literature summary extracted from

  • Lam, Y.; Nicholas, D.J.D.
    A nitrite reductase with cytochrome oxidase activity from Micrococcus denitrificans (1969), Biochim. Biophys. Acta, 180, 459-472.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.1 KCN 1 mM, 72% inhibition Paracoccus denitrificans
1.7.2.1 additional information relatively ineffective at 1 mM: CO, bathocuproine, diethyl dithiocarbamate, o-phenanthroline and alpha,alpha'-dipyridyl Paracoccus denitrificans
1.7.2.1 NaN3 1 mM, 60% inhibition Paracoccus denitrificans
1.7.2.1 NH2OH 1 mM, 50% inhibition Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.1 0.027
-
 O2 cytochrome c oxidase activity Paracoccus denitrificans
1.7.2.1 0.046
-
 ferricytochrome c
-
 Paracoccus denitrificans
1.7.2.1 0.046
-
 NO2- reduced cytochrome c as electron donor Paracoccus denitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper copper protein Paracoccus denitrificans
1.7.2.1 copper 5.5 ng/mg protein Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.1 130000
-
 gel filtration Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.1 NO2- + ferrocytochrome c Paracoccus denitrificans role in respiration NO + ferricytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Paracoccus denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.1
-
 Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.1 1.27
-
-
 Paracoccus denitrificans

Storage Stability

EC Number Storage Stability Organism
1.7.2.1 -15°C, 4 weeks Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 ferrocytochrome c + O2
-
 Paracoccus denitrificans ferricytochrome c + H2O
-
 ?
1.7.2.1 nitric oxide + H2O + ferricytochrome c
-
 Paracoccus denitrificans nitrite + ferrocytochrome c + H+
-
 r
1.7.2.1 nitrite + ferrocytochrome c artificial electron donors: reduced methyl viologen, phenazine methosulfate and to a lesser extend hydroquinone, highly purified enzyme has cytochrome c oxidase activity Paracoccus denitrificans nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 nitrite + ferrocytochrome c artificial electron donor: reduced benzyl viologen Paracoccus denitrificans nitric oxide + H2O + ferricytochrome c
-
 ?
1.7.2.1 NO2- + ferrocytochrome c role in respiration Paracoccus denitrificans NO + ferricytochrome c
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.2.1 30
-
-
 Paracoccus denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.1 6.5 7 cytochrome c oxidase activity Paracoccus denitrificans
1.7.2.1 6.7
-
-
 Paracoccus denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.1 cytochrome contains cytochrome, probably of the c and a2 types Paracoccus denitrificans