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Literature summary extracted from

  • Reineke, W.; Knackmuss, H.J.
    Chemical structure and biodegradability of halogenated aromatic compounds. Substituent effects on dehydrogenation of 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid (1978), Biochim. Biophys. Acta, 542, 424-429.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.25 additional information
-
 additional information substituted substrates Pseudomonas sp.
1.3.1.25 additional information
-
 additional information substituted substrates Cupriavidus necator
1.3.1.25 0.15
-
 NAD+ pH 8.0 Cupriavidus necator
1.3.1.25 0.67
-
 NAD+
-
 Pseudomonas sp.
1.3.1.25 1
-
 NAD+ pH 9.6 Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas sp. free carboxyl groups essential catechol + CO2 + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Cupriavidus necator free carboxyl groups essential catechol + CO2 + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas sp. B13 free carboxyl groups essential catechol + CO2 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.25 Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-
1.3.1.25 Pseudomonas sp.
-
-
-
1.3.1.25 Pseudomonas sp. B13
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.25
-
 Pseudomonas sp.
1.3.1.25
-
 Cupriavidus necator

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.25 (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+ mechanism Pseudomonas sp.
a
1.3.1.25 (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+ mechanism Cupriavidus necator
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.25 14.4
-
-
 Cupriavidus necator
1.3.1.25 39.4 59.8
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ free carboxyl groups essential Pseudomonas sp. catechol + CO2 + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ free carboxyl groups essential Cupriavidus necator catechol + CO2 + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ free carboxyl groups essential Pseudomonas sp. B13 catechol + CO2 + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.25 8
-
-
 Cupriavidus necator
1.3.1.25 8.4
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.25 NAD+
-
 Pseudomonas sp.
1.3.1.25 NAD+
-
 Cupriavidus necator