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Literature summary extracted from

  • Reineke, W.; Knackmuss, H.J.
    Hybrid pathway for chlorobenzoate metabolism in Pseudomonas sp. B13 derivatives (1980), J. Bacteriol., 142, 467-473.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.25 addition of recombinant enzyme on plasmid TOL plasmid of donor PaW1 to native one in B13 derivative WR1 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas sp.
-
 catechol + CO2 + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ Pseudomonas sp. B13
-
 catechol + CO2 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.25 Pseudomonas sp.
-
-
-
1.3.1.25 Pseudomonas sp. B13
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.25 (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+ by growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate enzyme is induced when combinated with recombinant toluate-1,2-dioxygenase Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.25 additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
 Pseudomonas sp. catechol + CO2 + NADH
-
 ?
1.3.1.25 (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
-
 Pseudomonas sp. B13 catechol + CO2 + NADH
-
 ?
1.3.1.25 3-methyldihydrodihydroxybenzoate + NAD+ assay substrate Pseudomonas sp. 3-methylcatechol + CO2 + NADH
-
 ?
1.3.1.25 3-methyldihydrodihydroxybenzoate + NAD+ assay substrate Pseudomonas sp. B13 3-methylcatechol + CO2 + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.25 50
-
 native enzyme: 10 min, 90% activity, recombinant enzyme: 5 min, complete inactivation Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.25 NAD+
-
 Pseudomonas sp.