EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.25 | expression of wild-type benD gene and native mutant genes in Escherichia coli and Acinetobacter calcoaceticus | Acinetobacter calcoaceticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.25 | 31000 | - |
2 * 31000, SDS-PAGE | Acinetobacter calcoaceticus |
1.3.1.25 | 62000 | - |
gel filtration | Acinetobacter calcoaceticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.25 | (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | Acinetobacter calcoaceticus | enzyme in the pathway of dissimilation of benzoate via catechol | catechol + CO2 + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.25 | Acinetobacter calcoaceticus | - |
wild-type ADP1 and spontaneous mutants ADP136, ADP137, ADP138 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.25 | partial, recombinant from Escherichia coli and Acinetobacter calcoaceticus | Acinetobacter calcoaceticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.25 | additional information | - |
wild-type enzyme, native and recombinant, and mutants, activity depending on growth substrates | Acinetobacter calcoaceticus |
1.3.1.25 | 108 | - |
- |
Acinetobacter calcoaceticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.25 | (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | - |
Acinetobacter calcoaceticus | catechol + CO2 + NADH | - |
? | |
1.3.1.25 | (-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+ | enzyme in the pathway of dissimilation of benzoate via catechol | Acinetobacter calcoaceticus | catechol + CO2 + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.25 | dimer | 2 * 31000, SDS-PAGE | Acinetobacter calcoaceticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.25 | DHB dehydrogenase | - |
Acinetobacter calcoaceticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.25 | NAD+ | - |
Acinetobacter calcoaceticus |