EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.6 | additional information | enzyme is induced during glycolysis | Pyrococcus furiosus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.7.6 | DNA sequence determination and analysis | Pyrococcus furiosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.6 | Fe2+ | enzyme contains iron-sulfur cluster | Pyrococcus furiosus | |
1.2.7.6 | Tungsten | single tungsten environment is afforded for the reaction | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.6 | 56000 | - |
gel filtration | Pyrococcus furiosus |
1.2.7.6 | 64000 | - |
1 * 64000, SDS-PAGE | Pyrococcus furiosus |
1.2.7.6 | 74000 | - |
sequence determination | Pyrococcus furiosus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.6 | D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | Pyrococcus furiosus | enzyme is a site for glycolytic regulation | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.6 | Pyrococcus furiosus | Q8U3K2 | - |
- |
1.2.7.6 | Pyrococcus furiosus | Q8U3K2 | hyperthermophilic archaeon | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.6 | 35fold, about 95% purity | Pyrococcus furiosus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.2.7.6 | D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin = 3-phospho-D-glycerate + 2 H+ + reduced ferredoxin | this enzyme is thought to function in place of glyceraldehyde-3-phosphate dehydrogenase and possibly phosphoglycerate kinase in the novel Embden-Meyerhoff-type glycolytic pathway found in Pyrococcus furiosus. It is specific for glyceraldehyde-3-phosphate | Pyrococcus furiosus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.7.6 | 25 | - |
purified enzyme | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.6 | D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen | artificial electron acceptor | Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced benzyl viologen | - |
? | |
1.2.7.6 | D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | - |
Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? | |
1.2.7.6 | D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin | enzyme is a site for glycolytic regulation | Pyrococcus furiosus | 3-phospho-D-glycerate + H+ + reduced ferredoxin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.6 | monomer | 1 * 64000, SDS-PAGE | Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.6 | glyceraldehyde-3-phosphate ferredoxin oxidoreductase | - |
Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.6 | 50 | - |
assay at | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.6 | 8 | - |
assay at | Pyrococcus furiosus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.6 | Ferredoxin | dependent on | Pyrococcus furiosus |