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Literature summary extracted from

  • Chan, M.K.; Mukund, S.; Kletzin, A.; Adams, M.W.; Rees, D.C.
    Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase (1995), Science, 267, 1463-1469.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.7.5 X-ray crystal structure determination and analysis Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.5 Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Pyrococcus furiosus
1.2.7.5 Molybdenum part of a tungsten-molybdopterin cofactor Pyrococcus furiosus
1.2.7.5 Tungsten part of a tungsten-molybdopterin cofactor Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.5 66000
-
 2 * 66000, crystal structure Pyrococcus furiosus
1.2.7.5 134000
-
 crystal structure Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus
-
 an acid + H+ + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.5 Pyrococcus furiosus
-
 hyperthermophilic archaeon
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin = a carboxylate + 2 H+ + reduced ferredoxin substrate binding, catalytic site and reaction mechanism including cofactor action Pyrococcus furiosus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.7.5 dimer 2 * 66000, crystal structure Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.5 95
-
 above Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.5 additional information
-
 the enzyme is thermostable Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.5 Ferredoxin
-
 Pyrococcus furiosus
1.2.7.5 tungsten-molybdopterin tungstopterin center Pyrococcus furiosus
1.2.7.5 tungsten-molybdopterin structural coordination of the tungsten by 4 sulfur ligands in the cofactor Pyrococcus furiosus
1.2.7.5 tungsten-molybdopterin interaction with of DXXGL(D,C)X motif and Gly341, Asp343, Asp338, Gly492, Cys494, and Asp489 Pyrococcus furiosus