EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.B2 | arsenite | 5 mM, 50% inhibition after min | Thermococcus litoralis | |
1.2.7.B2 | cyanide | 5 mM, 50% inhibition after min | Thermococcus litoralis | |
1.2.7.B2 | iodoacetate | 5 mM, 50% inhibition after min | Thermococcus litoralis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.B2 | Iron | the enzyme contains 16.0 gatom of iron per 280000 Da g of enzyme. Each subunit of dithionite reduced enzyme contains a magnetically isolated [4Fe-4S]1+ cluster that exists in both the S = 3/2 and S = 1/2 states | Thermococcus litoralis | |
1.2.7.B2 | Tungsten | tungsten-containing enzyme. The enzyme contains 3.3 gatom of tungsten per 280000 Da g of enzyme | Thermococcus litoralis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.B2 | 30000 | - |
gel filtration | Thermococcus litoralis |
1.2.7.B2 | 70000 | - |
4 * 70000, SDS-PAGE | Thermococcus litoralis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.B2 | Thermococcus litoralis | Q56303 | - |
- |
1.2.7.B2 | Thermococcus litoralis DSM 5473 | Q56303 | - |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.2.7.B2 | the sensitivity to inactivation by O2, of formaldehyde ferredoxin oxidoreductase in the purified state is similar to that of the cell-free extract. That is, when the enzyme (5.0 mg/ml) as isolated under reducing conditions is briefly shaken in air to oxidize the sodium dithionite and then left exposed at 23°C, 50% of the original activity is lost after 5 h | Thermococcus litoralis |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.B2 | - |
Thermococcus litoralis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.7.B2 | 6.8 | - |
pH 8.4, 80°C | Thermococcus litoralis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.B2 | acetaldehyde + H2O + oxidized methyl viologen | - |
Thermococcus litoralis | acetate + H+ + reduced methyl viologen | - |
? | |
1.2.7.B2 | acetaldehyde + H2O + oxidized methyl viologen | - |
Thermococcus litoralis DSM 5473 | acetate + H+ + reduced methyl viologen | - |
? | |
1.2.7.B2 | butyraldehyde + H2O + oxidized methyl viologen | 10% compared to the activity with acetyladehyde | Thermococcus litoralis | butyrate + H+ + reduced methyl viologen | - |
? | |
1.2.7.B2 | butyraldehyde + H2O + oxidized methyl viologen | 10% compared to the activity with acetyladehyde | Thermococcus litoralis DSM 5473 | butyrate + H+ + reduced methyl viologen | - |
? | |
1.2.7.B2 | formaldehyde + H2O + 2 oxidized ferredoxin | - |
Thermococcus litoralis | formate + 2 H+ + 2 reduced ferredoxin | - |
? | |
1.2.7.B2 | formaldehyde + H2O + 2 oxidized ferredoxin | - |
Thermococcus litoralis DSM 5473 | formate + 2 H+ + 2 reduced ferredoxin | - |
? | |
1.2.7.B2 | formaldehyde + H2O + oxidized methyl viologen | 63% compared to the activity with acetyladehyde | Thermococcus litoralis | formate + H+ + reduced methyl viologen | - |
? | |
1.2.7.B2 | formaldehyde + H2O + oxidized methyl viologen | 63% compared to the activity with acetyladehyde | Thermococcus litoralis DSM 5473 | formate + H+ + reduced methyl viologen | - |
? | |
1.2.7.B2 | additional information | activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde | Thermococcus litoralis | ? | - |
? | |
1.2.7.B2 | additional information | activity with crotonaldehyde is less than 10% compared to activity with acetaldehyde: crotonaldehyde, benzaldehyde, phenylacetaldehyde | Thermococcus litoralis DSM 5473 | ? | - |
? | |
1.2.7.B2 | propionaldehyde + H2O + oxidized methyl viologen | 77% compared to the activity with acetyladehyde | Thermococcus litoralis | propionate + H+ + reduced methyl viologen | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.B2 | tetramer | 4 * 70000, SDS-PAGE | Thermococcus litoralis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.B2 | FOR | - |
Thermococcus litoralis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.B2 | 80 | - |
assay at | Thermococcus litoralis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.B2 | 80 | - |
2 h, 50% loss of activity | Thermococcus litoralis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.B2 | 8.4 | - |
assay at | Thermococcus litoralis |