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Literature summary extracted from

  • Woolfolk, C.A.; Downard, J.S.
    Bacterial xanthine oxidase from Arthrobacter S-2 (1978), J. Bacteriol., 135, 422-428.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.17.3.2 stable for more than 1 year with alternate freezing and thawing Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.2 xanthine no substrate inhibition Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.3.2 additional information
-
 additional information
-
 Arthrobacter sp.
1.17.3.2 0.13
-
 xanthine
-
 Arthrobacter sp.
1.17.3.2 0.13
-
 1-Methylxanthine
-
 Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.3.2 Iron iron-molybdenum protein Arthrobacter sp.
1.17.3.2 Molybdenum an iron-molybdenum protein Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.3.2 80000
-
 2 * 80000, SDS-PAGE Arthrobacter sp.
1.17.3.2 146000
-
 gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.17.3.2 Arthrobacter sp.
-
 S-2
-
1.17.3.2 Arthrobacter sp. S-2
-
 S-2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.17.3.2 additional information
-
 Arthrobacter sp.
1.17.3.2 proteolytic modification
-
 Arthrobacter sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.3.2
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.3.2 230
-
-
 Arthrobacter sp.

Storage Stability

EC Number Storage Stability Organism
1.17.3.2 -75°C, more than 1 year with alternate freezing and thawing, stable Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.3.2 1-methylxanthine + H2O + O2
-
 Arthrobacter sp. 1-methylurate + H2O2
-
 ?
1.17.3.2 hypoxanthine + 2 H2O + 2 O2
-
 Arthrobacter sp. urate + 2 H2O2
-
 ?
1.17.3.2 hypoxanthine + 2 H2O + 2 O2
-
 Arthrobacter sp. S-2 urate + 2 H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 electron acceptor O2 Arthrobacter sp. uric acid + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 Arthrobacter S-2 enzyme: relatively specific Arthrobacter sp. uric acid + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 electron acceptor ferricyanide Arthrobacter sp. uric acid + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 electron acceptor 2,6-dichlorophenolindophenol Arthrobacter sp. uric acid + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 electron acceptor O2 Arthrobacter sp. S-2 uric acid + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 Arthrobacter S-2 enzyme: relatively specific Arthrobacter sp. S-2 uric acid + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 electron acceptor ferricyanide Arthrobacter sp. S-2 uric acid + H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 electron acceptor 2,6-dichlorophenolindophenol Arthrobacter sp. S-2 uric acid + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.3.2 dimer 2 * 80000, SDS-PAGE Arthrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.3.2 FAD flavoprotein Arthrobacter sp.