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Literature summary extracted from

  • Kaminsky, Z.W.; Jezewska, M.M.
    Involvement of a single thiol group in the conversion of the NAD+-dependent activity of rat liver xanthine oxidoreductase to the O2-dependent activity (1982), Biochem. J., 207, 341-346.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.4 4-chloromercuribenzoate increases total activity up to 0.005 mM Rattus sp.

Application

EC Number Application Comment Organism
1.17.1.4 medicine modification of xanthine oxidoreductase by disulfiram that is used in the management of chronic alcolism Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 2-Iodosobenzoic acid 50% inhibition at 0.0025 mM of NAD+-dependent activity by enzyme inactivation, not by conversion to the O2-dependent activity Rattus sp.
1.17.1.4 4-chloromercuribenzoate decreases NAD+-dependent activity from 0.01 up to 0.05 mM with simultaneous inactivation of the enzyme Rattus sp.
1.17.1.4 5,5-dithiobis-(2-nitrobenzoate) conversion from of NAD+-dependent to O2-dependent activity without any effect on the total activity Rattus sp.
1.17.1.4 NADH product inhibition Rattus sp.
1.17.1.4 tetraethyldithiodicarbonic diamide i.e. disulfiram; transformation of the NAD+- to the O2-dependent activity up to 0.025 mM, up to 80% loss of NAD+-dependent activity, modification of one thiol group in the active centre, NAD+ protects against modification due to a single thiol group involved in NAD+-binding within the active centre Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Rattus sp. NAD+-dependent form is postulated to play a regulatory role in purine metabolism urate + NADH
-
 ?
1.17.1.4 xanthine + O2 + H2O Rattus sp. NAD+-independent xanthine oxidase activity, low activity present in the enzyme preparation, conversion of the NAD+-dependent to NAD+-independent activity by some thiol reagents urate + O2- + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, mainly NAD+-dependent activity Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 liver
-
 Rattus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O NAD+-dependent form is postulated to play a regulatory role in purine metabolism Rattus sp. urate + NADH
-
 ?
1.17.1.4 xanthine + O2 + H2O NAD+-independent xanthine oxidase activity, low activity present in the enzyme preparation, conversion of the NAD+-dependent to NAD+-independent activity by some thiol reagents Rattus sp. urate + O2- + 2 H+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+
-
 Rattus sp.