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Literature summary extracted from

  • Hughes, R.K.; Bennett, B.; Bray, R.C.
    Xanthine dehydrogenase from Drosophila melanogaster: Purification and properties of the wild-type enzyme and of a variant lacking iron-sulfur centers (1992), Biochemistry, 31, 3073-3083.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.4 E89K natural mutant strain, lacking iron-sulfur centers, activity to xanthine/NAD+ or xanthine/pterin not affected, but xanthine/phenazine methosulfate activity abolished Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Iron-sulfur-center
-
Drosophila melanogaster
1.17.1.4 molybdenum-center
-
Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 2-amino-4-hydroxy-pterin + NAD+ + H2O Drosophila melanogaster i.e. pterin isoxanthopterin + NADH
-
?
1.17.1.4 xanthine + NAD+ + H2O Drosophila melanogaster
-
urate + NADH
-
?
1.17.1.4 xanthine + O2 + H2O Drosophila melanogaster
-
urate + O2- + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, chromatography techniques Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 whole body analysis of a rosy mutant strain Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 0.54
-
-
Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 2-amino-4-hydroxy-pterin + NAD+ + H2O i.e. pterin Drosophila melanogaster isoxanthopterin + NADH
-
?
1.17.1.4 2-amino-4-hydroxypterin + NAD+ + H2O i.e. pterin Drosophila melanogaster isoxanthopterin + NADH
-
?
1.17.1.4 NADH + electron acceptor + H2O electron acceptor: 2,6-dichlorophenolindophenol, no activity for mutant E89K Drosophila melanogaster NAD+ + reduced electron acceptor
-
?
1.17.1.4 NADH + reduced phenazine methosulfate + cytochrome c no activity for the mutant E89K Drosophila melanogaster NAD+ + ?
-
?
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O very low activity for the mutant E89K Drosophila melanogaster urate + ?
-
?
1.17.1.4 xanthine + NAD+ + H2O
-
Drosophila melanogaster urate + NADH
-
?
1.17.1.4 xanthine + O2 + H2O
-
Drosophila melanogaster urate + O2- + 2 H+
-
?
1.17.1.4 xanthine + phenazine methosulfate + cytochrome c + H2O very low activity for the mutant E89K Drosophila melanogaster urate + ?
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.4 12.2
-
xanthine electron acceptor: NAD+ Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD
-
Drosophila melanogaster
1.17.1.4 additional information
-
Drosophila melanogaster
1.17.1.4 NAD+
-
Drosophila melanogaster