Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Perez-Vicente, R.; Alamillo, J.M.; Cardenas, J.; Pineda, M.
    Purification and substrate inactivation of xanthine dehydrogenase from Chlamydomonas reinhardtii (1992), Biochim. Biophys. Acta, 1117, 159-166.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 8-Azaadenine competitive inhibition of xanthine oxidation, Ki: 0.25 mM Chlamydomonas reinhardtii
1.17.1.4 acetaldehyde inactivation Chlamydomonas reinhardtii
1.17.1.4 adenine competitive inhibition of xanthine oxidation, Ki: 0.13 mM Chlamydomonas reinhardtii
1.17.1.4 hypoxanthine inactivation of xanthine oxidase activity, not in the presence of NAD+ Chlamydomonas reinhardtii
1.17.1.4 NADH inactivation closely related to associated diaphorase activity Chlamydomonas reinhardtii
1.17.1.4 NADPH inactivation closely related to diaphorase activity Chlamydomonas reinhardtii
1.17.1.4 Sodium dithionite irreversible inactivation by reduction of xanthine dehydrogenase, no recovery after dithionite elimination Chlamydomonas reinhardtii
1.17.1.4 xanthine irreversible inactivation by reduction of xanthine dehydrogenase; irreversible inhibition of xanthine oxidase activity, adenine and 8-azaadenine protects against inactivation, ferricyanide partially protects against inactivation, no inactivation in the presence of NAD+ Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H2O Chlamydomonas reinhardtii
-
 xanthine + NADH + H+
-
 ?
1.17.1.4 hypoxanthine + NADP+ + H2O Chlamydomonas reinhardtii
-
 xanthine + NADPH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O Chlamydomonas reinhardtii
-
 urate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, chromatography, preparative gel electrophoresis Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture
-
 Chlamydomonas reinhardtii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 5.69
-
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 8-azaxanthine + NAD+ + H2O very low activity Chlamydomonas reinhardtii 8-aza-urate + NADH
-
 ?
1.17.1.4 acetaldehyde + NAD+ + H2O very low activity Chlamydomonas reinhardtii acetate + NADH + H+
-
 ?
1.17.1.4 adenine + NAD+ + H2O low activity Chlamydomonas reinhardtii ? + NADH
-
 ?
1.17.1.4 hypoxanthine + NAD+ + H2O
-
 Chlamydomonas reinhardtii xanthine + NADH + H+
-
 ?
1.17.1.4 hypoxanthine + NADP+ + H2O
-
 Chlamydomonas reinhardtii xanthine + NADPH
-
 ?
1.17.1.4 NADH + electron acceptor + H2O electron acceptor: nitroblue tetrazolium Chlamydomonas reinhardtii NAD+ + reduced electron acceptor
-
 ?
1.17.1.4 NADPH + nitroblue tetrazolium + H2O diaphorase activity Chlamydomonas reinhardtii NADP+ + reduced nitroblue tetrazolium
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Chlamydomonas reinhardtii urate + NADH
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+
-
 Chlamydomonas reinhardtii
1.17.1.4 NADP+
-
 Chlamydomonas reinhardtii