Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gremer, L.; Meyer, O.
    Characterization of xanthine dehydrogenase from the anaerobic bacterium Veillonella atypica and identification of molybdopterin-cytosine-dinucleotide-containing molybdenum cofactor (1996), Eur. J. Biochem., 238, 862-866.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.017
-
xanthine electron acceptor: ferricyanide Veillonella atypica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Iron-sulfur-center 1 Fe2-S2-cluster Veillonella atypica
1.17.1.4 molybdenum-center molybdopterin cytosine dinucleotide cofactor Veillonella atypica
1.17.1.4 Zn 1.54 mol Zn per mol enzyme Veillonella atypica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 18400
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE Veillonella atypica
1.17.1.4 28500
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE Veillonella atypica
1.17.1.4 82400
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE Veillonella atypica
1.17.1.4 129000
-
native PAGE Veillonella atypica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + O2 + H2O Veillonella atypica very low activity urate + O2- + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Veillonella atypica
-
formerly Micrococcus lactilyticus or Veillonella alcalescens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, chromatography techniques Veillonella atypica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture inducible by xanthine Veillonella atypica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 72.56
-
xanthine-ferricyanide assay Veillonella atypica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 acetaldehyde + ferricyanide + H2O 5% of the activity compared to xanthine Veillonella atypica acetic acid + ferrocyanide
-
?
1.17.1.4 adenine + ferricyanide + H2O 12% of the activity compared to xanthine Veillonella atypica urate + ferrocyanide
-
?
1.17.1.4 benzaldehyde + 2 ferricyanide + H2O 4% of the activity compared to xanthine Veillonella atypica benzoate + 2 ferrocyanide + 2 H+
-
?
1.17.1.4 hypoxanthine + ferricyanide + H2O 98% of the activity compared to xanthine Veillonella atypica xanthine + ferrocyanide
-
?
1.17.1.4 purine + ferricyanide + H2O 108% of the activity compared to xanthine Veillonella atypica urate + ferrocyanide
-
?
1.17.1.4 salicylaldehyde + ferricyanide + H2O 2% of the activity compared to xanthine Veillonella atypica salicylate + ferrocyanide
-
?
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O 13% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + ?
-
?
1.17.1.4 xanthine + benzyl viologen + H2O 18% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + reduced benzyl viologen
-
?
1.17.1.4 xanthine + cytochrome c + H2O low activity Veillonella atypica urate + reduced cytochrome c
-
?
1.17.1.4 xanthine + FAD + H2O 35% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + FADH2
-
?
1.17.1.4 xanthine + ferricyanide + H2O
-
Veillonella atypica urate + ferrocyanide
-
?
1.17.1.4 xanthine + FMN + H2O 44% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + FMNH2
-
?
1.17.1.4 xanthine + iodonitrotetrazolium + H2O
-
Veillonella atypica urate + reduced iodonitrotetrazolium
-
?
1.17.1.4 xanthine + methyl viologen + H2O low activity Veillonella atypica urate + reduced methyl viologen
-
?
1.17.1.4 xanthine + methylene blue + H2O
-
Veillonella atypica urate + reduced methylene blue
-
?
1.17.1.4 xanthine + nitroblue tetrazolium + H2O
-
Veillonella atypica urate + ?
-
?
1.17.1.4 xanthine + O2 + H2O very low activity Veillonella atypica urate + O2- + 2 H+
-
?
1.17.1.4 xanthine + phenazine methosulfate + H2O
-
Veillonella atypica urate + ?
-
?
1.17.1.4 xanthine + riboflavin + H2O 41% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + reduced riboflavin
-
?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 trimer 1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE Veillonella atypica

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD
-
Veillonella atypica
1.17.1.4 additional information molybdoironflavoprotein: molar ratio of molybdenum to iron to acid-labile-sulfur to FAD is 1 : 2 : 1.9 : 0.8 Veillonella atypica