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Literature summary extracted from

  • Parschat, K.; Canne, C.; Huttermann, J.; Kappl, R.; Fetzner, S.
    Xanthine dehydrogenase from Pseudomonas putida 86: Specificity, oxidation-reduction potentials of its redox-active centers, and first EPR characterization (2001), Biochim. Biophys. Acta, 1544, 151-165.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Iron contains FeSI and FeSII clusters Pseudomonas putida
1.17.1.4 Iron-sulfur-center
-
Pseudomonas putida
1.17.1.4 Molybdenum contains molybdenum-molybdopterin Pseudomonas putida
1.17.1.4 molybdenum-center
-
Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 46238
-
2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry Pseudomonas putida
1.17.1.4 51000
-
2 * 94000 + 2 * 51000, SDS-PAGE Pseudomonas putida
1.17.1.4 91041
-
2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry Pseudomonas putida
1.17.1.4 94000
-
2 * 94000 + 2 * 51000, SDS-PAGE Pseudomonas putida
1.17.1.4 580000
-
SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H2O Pseudomonas putida
-
xanthine + NADH + H+
-
?
1.17.1.4 xanthine + NAD+ + H2O Pseudomonas putida
-
urate + NADH
-
?
1.17.1.4 xanthine + O2 + H2O Pseudomonas putida 4% of the activity compared to xanthine-NAD+ urate + O2- + 2 H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Pseudomonas putida
-
-
-
1.17.1.4 Pseudomonas putida 86
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4
-
Pseudomonas putida
1.17.1.4 ammonium sulfate precipitation, phenyl-Sepharose column chromatography, DEAE-10 Macro-Prep MP10 column chromatography, and Bio-Prep SE-1000/17 column chromatography Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture inducible by hypoxanthine Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 0.9
-
crude extract Pseudomonas putida
1.17.1.4 26.7
-
hypoxanthine-NAD+ assay Pseudomonas putida
1.17.1.4 35.2
-
after 39.1fold purification Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 3,4-dihydroxybenzaldehyde + NAD+ + H2O 1.2% activity compared to xanthine Pseudomonas putida 3,4-dihydroxybenzoate + NADH + H+
-
?
1.17.1.4 3,4-dihydroxybenzaldehyde + NAD+ + H2O 1.2% activity compared to xanthine Pseudomonas putida 86 3,4-dihydroxybenzoate + NADH + H+
-
?
1.17.1.4 4-dimethylaminobenzaldehyde + NAD+ + H2O 0.3% activity compared to xanthine Pseudomonas putida 4-dimethylaminobenzoate + NADH
-
?
1.17.1.4 4-hydroxybenzaldehyde + NAD+ + H2O 0.7% activity compared to xanthine Pseudomonas putida 4-hydroxybenzoate + NADH + H+
-
?
1.17.1.4 4-hydroxybenzaldehyde + NAD+ + H2O 0.7% activity compared to xanthine Pseudomonas putida 86 4-hydroxybenzoate + NADH + H+
-
?
1.17.1.4 benzaldehyde + NAD+ + H2O 1.3% activity compared to xanthine Pseudomonas putida benzoate + NADH + H+
-
?
1.17.1.4 benzaldehyde + NAD+ + H2O 1.3% activity compared to xanthine Pseudomonas putida 86 benzoate + NADH + H+
-
?
1.17.1.4 hypoxanthine + ferricyanide + H2O
-
Pseudomonas putida xanthine + ferrocyanide
-
?
1.17.1.4 hypoxanthine + ferricyanide + H2O
-
Pseudomonas putida 86 xanthine + ferrocyanide
-
?
1.17.1.4 hypoxanthine + NAD+ + H2O
-
Pseudomonas putida xanthine + NADH + H+
-
?
1.17.1.4 hypoxanthine + NAD+ + H2O 149% activity compared to xanthine Pseudomonas putida urate + ? + NADH
-
?
1.17.1.4 additional information NAD+ is the most effcient electron acceptor, followed by 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and ferricyanide Pseudomonas putida ?
-
?
1.17.1.4 additional information NAD+ is the most effcient electron acceptor, followed by 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride and ferricyanide Pseudomonas putida 86 ?
-
?
1.17.1.4 phthalazine + NAD+ + H2O 0.4% activity compared to xanthine Pseudomonas putida 1-(2H)-phthalazinone + NADH
-
?
1.17.1.4 purine + NAD+ + H2O 5% activity compared to xanthine Pseudomonas putida 8-hydroxypurine + NADH + H+
-
?
1.17.1.4 quinazoline + NAD+ + H2O 2% activity compared to xanthine Pseudomonas putida 4-(3H)-quinazolinone + NADH
-
?
1.17.1.4 xanthine + ferricyanide + H2O 58% of the activity compared to xanthine-NAD+ Pseudomonas putida urate + ferrocyanide
-
?
1.17.1.4 xanthine + NAD+ + H2O
-
Pseudomonas putida urate + NADH
-
?
1.17.1.4 xanthine + NAD+ + H2O 100% activity Pseudomonas putida urate + NADH
-
?
1.17.1.4 xanthine + O2 + H2O 4% of the activity compared to xanthine-NAD+ Pseudomonas putida urate + O2- + 2 H+
-
?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 heterooctamer 2 * 91041 + 2 * 46238, MALDI-TOF mass spectrometry Pseudomonas putida
1.17.1.4 heterooctamer 2 * 94000 + 2 * 51000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.17.1.4 XDH
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD
-
Pseudomonas putida
1.17.1.4 additional information cofactor compostion similar to eukaryotic enzymes Pseudomonas putida
1.17.1.4 NAD+
-
Pseudomonas putida
1.17.1.4 NADP+
-
Pseudomonas putida