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Literature summary extracted from

  • Volc, J.; Kubátová, E.; Daniel, G.; Sedmera, P.; Haltrich, D.
    Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes (2001), Arch. Microbiol., 176, 178-186.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.99.29 repeated freezing and thawing, two cycles, in the absence of glycerol has no effect on PDH activity Chlorophyllum rhacodes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.29 1.82
-
 D-glucose 25°C, ferricenium ion as electron acceptor Chlorophyllum rhacodes
1.1.99.29 24
-
 L-arabinose 25°C, ferricenium ion as electron acceptor Chlorophyllum rhacodes
1.1.99.29 29.2
-
 D-xylose 25°C, ferricenium ion as electron acceptor Chlorophyllum rhacodes
1.1.99.29 29.8
-
 D-galactose 25°C, ferricenium ion as electron acceptor Chlorophyllum rhacodes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.29 extracellular active extracellular secretion of PDH Chlorophyllum rhacodes
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.29 76000
-
 gel filtration Chlorophyllum rhacodes
1.1.99.29 78000
-
 1 * 78000, SDS-PAGE Chlorophyllum rhacodes

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.29 Chlorophyllum rhacodes
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.29 glycoprotein heavily glycosylated, the sugar moiety of PDH comprises 17.3% of its total molecular mass Chlorophyllum rhacodes

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.29 46fold Chlorophyllum rhacodes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.29 mycelium
-
 Chlorophyllum rhacodes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.29 67
-
 25°C, D-glucose and ferricenium ion as substrates Chlorophyllum rhacodes

Storage Stability

EC Number Storage Stability Organism
1.1.99.29 4°C, purified PDH, 20 mM sodium phosphate buffer, pH 7, 0.02% ProClin 300, 2 months, no loss of activity Chlorophyllum rhacodes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.29 2-deoxy-D-glucose + 1,4-benzoquinone 49% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 2-deoxy-D-glucose + ferricenium ion 21% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 3-dehydro-D-glucose + 1,4-benzoquinone
-
 Chlorophyllum rhacodes 2,3-didehydro-D-glucose + hydroquinone
-
 ?
1.1.99.29 a pyranoside + acceptor
-
 Chlorophyllum rhacodes a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor
-
 ?
1.1.99.29 cellobiose + 1,4-benzoquinone 13% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 cellobiose + ferricenium ion 7% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-galactose + 1,4-benzoquinone 66% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-galactose + ferricenium ion 60% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-glucono-1,5-lactone + 1,4-benzoquinone 20% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-glucono-1,5-lactone + ferricenium ion 8% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-glucose + 1,4-benzoquinone oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate Chlorophyllum rhacodes 3-dehydro-D-glucose + hydroquinone
-
 ?
1.1.99.29 D-glucose + ferricenium ion oxidizes D-glucose exclusively at C-3, D-glucose is the preferred electron donor susbtrate Chlorophyllum rhacodes 3-dehydro-D-glucose + ferrocene
-
 ?
1.1.99.29 D-lyxose + 1,4-benzoquinone 5% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-lyxose + ferricenium ion 5% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-mannose + 1,4-benzoquinone 32% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-mannose + ferricenium ion 26% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-xylose + 1,4-benzoquinone 72% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 D-xylose + ferricenium ion 81% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 L-arabinose + 1,4-benzoquinone 54% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 L-arabinose + ferricenium ion 72% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 L-glucose + ferricenium ion 11% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 maltose + 1,4-benzoquinone 64% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 maltose + ferricenium ion 45% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 maltotriose + 1,4-benzoquinone 21% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 maltotriose + ferricenium ion 14% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 methyl-alpha-D-galactopyranoside + 1,4-benzoquinone 11% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 methyl-alpha-D-galactopyranoside + ferricenium ion 8% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 methyl-alpha-D-glucopyranoside + 1,4-benzoquinone 141% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 methyl-alpha-D-glucopyranoside + ferricenium ion 143% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 methyl-beta-D-glucopyranoside + 1,4-benzoquinone 30% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 methyl-beta-D-glucopyranoside + ferricenium ion 17% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 additional information broad selectivity for sugar substrates, O2 does not act as oxidant, not: D-allose, D-altrose, D-arabinose, D-ribose, D-fructose, L-sorbose, lactose, D-arabinitol, dulcitol, D-mannitol Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 pyranose + acceptor
-
 Chlorophyllum rhacodes 2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor
-
 ?
1.1.99.29 sucrose + 1,4-benzoquinone 9% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 sucrose + ferricenium ion 6% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 trehalose + 1,4-benzoquinone 7% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?
1.1.99.29 trehalose + ferricenium ion 8% of the activity with D-glucose Chlorophyllum rhacodes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.29 monomer 1 * 78000, SDS-PAGE Chlorophyllum rhacodes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.29 65
-
 ferricenium ion as electron acceptor Chlorophyllum rhacodes
1.1.99.29 75
-
 1,4-benzoquinone as electron acceptor Chlorophyllum rhacodes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.29 3.5
-
 50 mM sodium citrate buffer, 1,4-benzoquinone as electron acceptor Chlorophyllum rhacodes
1.1.99.29 9.5
-
 50 mM glycine buffer, ferricenium ion as electron acceptor Chlorophyllum rhacodes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.29 FAD functions as the cofactor Chlorophyllum rhacodes
1.1.99.29 flavin flavoprotein, FAD as cofactor Chlorophyllum rhacodes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.99.29 Chlorophyllum rhacodes isoelectric focusing
-
 4.2