Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schubert, H.L.; Raux, E.; Wilson, K.S.; Warren, M.J.
    Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis (1999), Biochemistry, 38, 10660-10669.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.99.1.3 overexpressed in Escherichia coli BL21(DE3)pLys cells by cloning the gene into pET14b Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.99.1.3 hexagonal crystals are grown using the hanging-drop method, crystals are in space group P6(3)22 with cell dimensions a = b = 128.08 A, c = 85.44 A Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
4.99.1.3 H145A/H207A site-directed mutagenesis Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.99.1.3 sirohydrochlorin + Co2+ Salmonella enterica subsp. enterica serovar Typhimurium cobalamin branched biosynthetic pathway cobalt-sirohydrochlorin + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.3
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.3 precorrin-2 + Co2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium cobalt-precorrin-2 + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+ cobalamin branched biosynthetic pathway Salmonella enterica subsp. enterica serovar Typhimurium cobalt-sirohydrochlorin + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.99.1.3 anaerobic cobalt chelatase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.99.1.3 CbiK
-
 Salmonella enterica subsp. enterica serovar Typhimurium