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Literature summary extracted from

  • Kushibe, S.; Sashida, R.; Morimoto, Y.; Ohkishi, H.
    Purification and characterization of a di-D-fructofuranose 2',1;2,1'-dianhydride producing enzyme from Streptomyces sp. MCI-2524 (1993), Biosci. Biotechnol. Biochem., 57, 2054-2058.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.18 Cu2+
-
 Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.18 extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.18 additional information no or little affected by various cations Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.18 36000
-
 2 * 36000, SDS-PAGE Streptomyces sp.
4.2.2.18 70000
-
 gel filtration Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.18 Streptomyces sp.
-
-
-
4.2.2.18 Streptomyces sp. MCI-2524
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.18
-
 Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.18 culture medium
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.18 256
-
 purified enzyme Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.18 inulin cf. EC 2.4.1.200 Streptomyces sp. di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?
4.2.2.18 inulin cf. EC 2.4.1.200 Streptomyces sp. MCI-2524 di-D-fructofuranose 1,2':2,1' dianhydride + ? i.e. DFA I ?

Subunits

EC Number Subunits Comment Organism
4.2.2.18 dimer 2 * 36000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.18 55
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.18 65
-
 stable up to Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.18 6
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.18 3 9
-
 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.18 4.5
-
 stable Streptomyces sp.