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Literature summary extracted from

  • Raychaudhuri, A.; Tipton, P.A.
    Cloning and expression of the gene for soybean hydroxyisourate hydrolase. Localization and implications for function and mechanism (2002), Plant Physiol., 130, 2061-2068.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.17 cloned and expressed in Escherichia coli Glycine max

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.17
-
 Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.17 E199A site-directed mutagenesis, mutant devoid of detectable catalytic activity Glycine max
3.5.2.17 E408A site-directed mutagenesis, mutant devoid of detectable catalytic activity Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.17 0.02
-
 5-hydroxyisourate pH 7.5, 37°C, recombinant enzyme lacking the signal peptide Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.17 peroxisome
-
 Glycine max 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.17 63730
-
 calculated from cDNA sequence Glycine max
3.5.2.17 68000
-
 SDS-PAGE, gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.17 5-hydroxyisourate + H2O Glycine max
-
 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.17 Glycine max
-
-
-
3.5.2.17 Glycine max Q8S3J3 soybean, cv Williams
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.17 recombinant enzyme Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.17 5-hydroxyisourate + H2O
-
 Glycine max 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.17 HIUHase
-
 Glycine max
3.5.2.17 hydroxyisourate hydrolase
-
 Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.17 24
-
 5-hydroxyisourate pH 7.5, 37°C, recombinant enzyme lacking the signal peptide Glycine max