EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.26 | expression of His-tagged CobS in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.8.26 | His-tagged CobS expressed in Escherichia coli BL21(lambdaDE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.26 | adenosylcobinamide-GDP + alpha-ribazole | Salmonella enterica subsp. enterica serovar Typhimurium | - |
GMP + adenosylcobalamin | - |
? | |
2.7.8.26 | adenosylcobinamide-GDP + alpha-ribazole | Salmonella enterica subsp. enterica serovar Typhimurium | de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin | adenosylcobalamin + GMP | - |
? | |
3.1.3.73 | alpha-ribazole 5'-phosphate + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, last steps in adenosylcobalamin biosynthesis | alpha-ribazole + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.26 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
2.7.8.26 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
strains JE212 and JE2197 | - |
3.1.3.73 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
strains JE212 and JE2197 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.26 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.8.26 | nickel affinity chromatography, recombinant CobS | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.8.26 | 0.008 | - |
recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.26 | adenosylcobinamide-GDP + alpha-ribazole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | GMP + adenosylcobalamin | - |
? | |
2.7.8.26 | adenosylcobinamide-GDP + alpha-ribazole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobalamin + GMP | - |
? | |
2.7.8.26 | adenosylcobinamide-GDP + alpha-ribazole | de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin | Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobalamin + GMP | - |
? | |
3.1.3.73 | adenosylcobalamin 5'-phosphate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobalamin + phosphate | - |
? | |
3.1.3.73 | alpha-ribazole 5'-phosphate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | alpha-ribazole + phosphate | - |
? | |
3.1.3.73 | alpha-ribazole 5'-phosphate + H2O | de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, last steps in adenosylcobalamin biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | alpha-ribazole + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.26 | cobalamin synthase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.7.8.26 | CobS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
3.1.3.73 | CobC | - |
Salmonella enterica subsp. enterica serovar Typhimurium |