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Literature summary extracted from

  • Varin, L.; Ibrahim, R.K.
    Novel flavonol 3-sulfotransferase. Purification, kinetic properties, and partial amino acid sequence (1992), J. Biol. Chem., 267, 1858-1863.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.25 3',5'-diphosphoadenosine competitive with respect to 3'-phosphoadenylylsulfate, noncompetitive with respect to quercetin Flaveria chlorifolia
2.8.2.25 additional information EDTA, SH-reagents at 1 and 10 mM, e.g. p-chloromercuribenzoate, iodoacetate or iodoacetamide, are not inhibitory Flaveria chlorifolia
2.8.2.25 quercetin 3-sulfate noncompetitive with respect to quercetin or 3'-phosphoadenylylsulfate Flaveria chlorifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.25 0.00018
-
 3'-phosphoadenylylsulfate cosubstrate quercetin, pH 6.0 Flaveria chlorifolia
2.8.2.25 0.0002
-
 quercetin pH 6.0 Flaveria chlorifolia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.25 additional information no divalent cation required Flaveria chlorifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.25 34500
-
 1 * 34500, SDS-PAGE Flaveria chlorifolia
2.8.2.25 35000
-
 gel filtration Flaveria chlorifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.25 3'-phosphoadenylylsulfate + quercetin Flaveria chlorifolia
-
 adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.25 Flaveria chlorifolia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.25 partial Flaveria chlorifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.25 plant
-
 Flaveria chlorifolia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.25 3'-phosphoadenylylsulfate + isorhamnetin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + isorhamnetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + kaempferol
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + kaempferol 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + patuletin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + patuletin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + quercetin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + quercetin 3-sulfate
-
 ?
2.8.2.25 3'-phosphoadenylylsulfate + rhamnetin
-
 Flaveria chlorifolia adenosine 3',5'-bisphosphate + rhamnetin 3-sulfate
-
 ?
2.8.2.25 additional information quercetagetin, gossypetin, myricetin or galangin are no substrates Flaveria chlorifolia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.25 monomer 1 * 34500, SDS-PAGE Flaveria chlorifolia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.25 30
-
 assay at Flaveria chlorifolia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.25 6
-
 and pH 8.5, two maxima Flaveria chlorifolia
2.8.2.25 8.5
-
 and pH 6, two maxima Flaveria chlorifolia