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Literature summary extracted from

  • Potempa, J.; Dubin, A.; Travis, J.
    Staphylopain (1998), Handbook of Proteolytic Enzymes (Barrett, A. ; Rawlings, N. D. ; Woessner, J. F. eds. ), , 669-671.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.48 reducing thiol compounds dependent on Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.48 Ag+
-
 Staphylococcus aureus
3.4.22.48 alpha2-Macroglobulin from human Staphylococcus aureus
3.4.22.48 E-64 stochiometrical and irreversible inhibition Staphylococcus aureus
3.4.22.48 Hg2+
-
 Staphylococcus aureus
3.4.22.48 additional information no inhibition by human kininogens and cystatin C Staphylococcus aureus
3.4.22.48 phosphorylated cystatin alpha from rat skin Staphylococcus aureus
3.4.22.48 T-Kininogen from rat Staphylococcus aureus
3.4.22.48 Zn2+
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.48 0.5
-
 N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide pH 8.0-8.8 Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.48 extracellular culture supernatant Staphylococcus aureus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.48 additional information not affected by changes in ionic strength by changing NaCl concentration Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.48 13000
-
 gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.48 peptide + H2O Staphylococcus aureus broad specificity peptide proteolytically cleaved into fragments or single amino acids
-
 ?
3.4.22.48 protein + H2O Staphylococcus aureus broad specificity protein proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 protein + H2O Staphylococcus aureus enzyme may play a role in growth regulation protein proteolytically cleaved into peptide fragments
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.48 Staphylococcus aureus
-
 enzyme might be inducible by elastin in vivo
-
3.4.22.48 Staphylococcus aureus
-
 V8
-
3.4.22.48 Staphylococcus aureus V8
-
 V8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.48 native from culture supernatant Staphylococcus aureus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.48 culture supernatant
-
 Staphylococcus aureus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.48 alcohol dehydrogenase + H2O
-
 Staphylococcus aureus alcohol dehydrogenase proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 alcohol dehydrogenase + H2O
-
 Staphylococcus aureus V8 alcohol dehydrogenase proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 benzyl-Tyr-OEt + H2O
-
 Staphylococcus aureus ?
-
 ?
3.4.22.48 casein + H2O
-
 Staphylococcus aureus casein proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 casein + H2O
-
 Staphylococcus aureus V8 casein proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 elastin + H2O insoluble substrate Staphylococcus aureus elastin proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 hemoglobin + H2O
-
 Staphylococcus aureus hemoglobin proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 hemoglobin + H2O
-
 Staphylococcus aureus V8 hemoglobin proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide + H2O
-
 Staphylococcus aureus N-benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline
-
 ?
3.4.22.48 peptide + H2O broad specificity Staphylococcus aureus amino acids
-
 ?
3.4.22.48 peptide + H2O broad specificity Staphylococcus aureus V8 amino acids
-
 ?
3.4.22.48 peptide + H2O broad specificity Staphylococcus aureus peptide proteolytically cleaved into fragments or single amino acids
-
 ?
3.4.22.48 protein + H2O broad specificity Staphylococcus aureus peptide fragments
-
 ?
3.4.22.48 protein + H2O broad specificity Staphylococcus aureus V8 peptide fragments
-
 ?
3.4.22.48 protein + H2O broad specificity Staphylococcus aureus protein proteolytically cleaved into peptide fragments
-
 ?
3.4.22.48 protein + H2O enzyme may play a role in growth regulation Staphylococcus aureus protein proteolytically cleaved into peptide fragments
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.48 monomer 1 * 13000, SDS-PAGE Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.48 0.16
-
 N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide pH 8.0-8.8 Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.48 additional information
-
 pI: 9.4 Staphylococcus aureus
3.4.22.48 6.5
-
 substrate insoluble elastin Staphylococcus aureus
3.4.22.48 7.5
-
 substrate benzyl-Tyr-OEt Staphylococcus aureus
3.4.22.48 8 8.8 substrate casein or hemoglobin Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.48 0.00052
-
 E-64 pH 8.0-8.8 Staphylococcus aureus