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Literature summary extracted from

  • Damuni, Z.; Merryfield, M.L.; Humphreys, J.S.; Reed, L.J.
    Purification and properties of branched-chain alpha-keto acid dehydrogenase phosphatase from bovine kidney (1984), Proc. Natl. Acad. Sci. USA, 81, 4335-4338.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.52 poly(L-arginine) stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus
3.1.3.52 poly(L-lysine) stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus
3.1.3.52 protamine stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.52 acyl-CoA inhibition is partially reversed by Mg2+ Bos taurus
3.1.3.52 ADP 50% inhibition at 400 mM; inhibition is completely reversed by Mg2+ Bos taurus
3.1.3.52 ATP 50% inhibition at 200 mM; inhibition is completely reversed by Mg2+ Bos taurus
3.1.3.52 CDP 50% inhibition at 400 mM; inhibition is completely reversed by Mg2+ Bos taurus
3.1.3.52 CoA inhibition is not reversed by Mg2+ Bos taurus
3.1.3.52 CTP 50% inhibition at 250 mM; inhibition is completely reversed by Mg2+ Bos taurus
3.1.3.52 GDP 50% inhibition at 200 mM; inhibition is completely reversed by Mg2+ Bos taurus
3.1.3.52 GTP 50% inhibition at 60 mM; inhibition is completely reversed by Mg2+ Bos taurus
3.1.3.52 guanosine 5'-(beta,gamma-imido)triphosphate
-
 Bos taurus
3.1.3.52 heparin 50% inhibition at 0.002 mg/ml, partially reversed by Mg2+ Bos taurus
3.1.3.52 additional information little inhibitory effect: AMP; little inhibitory effect: GMP; little inhibitory effect: NAD+; little inhibitory effect: NADH; little inhibitory effect: UMP Bos taurus
3.1.3.52 UDP 50% inhibition at 250 mM; inhibition is completely reversed by Mg2+ Bos taurus
3.1.3.52 UTP 50% inhibition at 100 mM; inhibition is completely reversed by Mg2+ Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.52 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.52 additional information active in absence of divalent cation Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.52 230000
-
 gel filtration, under conditions of high dilution, low MW form Bos taurus
3.1.3.52 460000
-
 gel filtration Bos taurus
3.1.3.52 460000
-
 high MW form Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.52 [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O Bos taurus
-
 [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.52 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.52 a high and a low MW form Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.52 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.52 0.24
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.1.3.52 4°C, partially purified enzyme stable for several weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.52 [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O
-
 Bos taurus [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.52 7 7.3
-
 Bos taurus