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Literature summary extracted from

  • Rigo, L.U.; Marechal, L.R.; Vieira, M.M.; Veiga, L.A.
    Oxidative pathway for L-rhamnose degradation in Pullularia pullulans (1985), Can. J. Microbiol., 31, 817-822.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.65 L-rhamnono-1,4-lactone + H2O Pullularia pullulans
-
 L-rhamnonate
-
 r
4.2.1.90 L-rhamnonate Aureobasidium pullulans enzyme of an oxidative pathway in the degradation of L-rhamnose 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.65 Pullularia pullulans
-
 yeastlike fungus, synonym Aureobasidium pullulans
-
4.2.1.90 Aureobasidium pullulans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.90 partial Aureobasidium pullulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.65 0.0013
-
 D-glucose as carbon source Pullularia pullulans
3.1.1.65 0.0017
-
 L-fucose as carbon source Pullularia pullulans
3.1.1.65 0.005
-
 glycerol as carbon source Pullularia pullulans
3.1.1.65 0.17
-
 L-rhamnose as carbon source Pullularia pullulans
4.2.1.90 0.17
-
-
 Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
 Pullularia pullulans L-rhamnonate
-
 r
4.2.1.90 L-rhamnonate
-
 Aureobasidium pullulans 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate enzyme of an oxidative pathway in the degradation of L-rhamnose Aureobasidium pullulans 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 additional information L-arabonate, D-gluconate, L-fuconate or D-galactonate at 0.002 or 0.02 M are no substrates Aureobasidium pullulans ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.90 30
-
 assay at Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.90 7
-
 assay at Aureobasidium pullulans