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Literature summary extracted from

  • Deana, R.; Rigoni, F.; Donella Deana, A.; Galzigna, L.
    Submitochondrial localization and partial purification of the succinylCoA: 3-hydroxy-3-methylglutarate coenzyme A transferase from rat liver (1981), Biochim. Biophys. Acta, 662, 119-124.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.3.13 2-mercaptoethanol increases activity Rattus norvegicus

General Stability

EC Number General Stability Organism
2.8.3.13 addition of 3mg/ml bovine serum albumin slightly increases stability, 10% glycerol stabilizes Rattus norvegicus
2.8.3.13 mercaptoethanol improves stability Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.13 acetoacetate non-competitive inhibition Rattus norvegicus
2.8.3.13 malonate competitive inhibition Rattus norvegicus
2.8.3.13 succinate competitive inhibition of the product Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.13 0.22
-
 succinyl-CoA pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate Rattus norvegicus
2.8.3.13 0.37
-
 malonyl-CoA pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate Rattus norvegicus
2.8.3.13 1.7
-
 3-hydroxy-3-methylglutarate pH 8.5, 30°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.3.13 mitochondrion mainly in the mitochondrial matrix, also in the inner membrane Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.13 42000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.13 succinyl-CoA + 3-hydroxy-3-methylglutarate Rattus norvegicus
-
 succinate + 3-hydroxy-3-methylglutaryl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.13 Rattus norvegicus
-
 Wistar albino
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.13 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.3.13 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.13 0.0194
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.3.13 -20°C, enzyme preparation with glycerol and mercaptoethanol retains 80% of its activity after 2 week of storage Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.13 butyryl-CoA + 3-hydroxy-3-methylglutarate only negligible amounts of HMGCoA produced Rattus norvegicus butyrate + 3-hydroxy-3-methylglutaryl-CoA
-
 ?
2.8.3.13 malonyl-CoA + 3-hydroxy-3-methylglutarate
-
 Rattus norvegicus malonate + 3-hydroxy-3-methylglutaryl-CoA
-
 r
2.8.3.13 succinyl-CoA + 3-hydroxy-3-methylglutarate
-
 Rattus norvegicus succinate + 3-hydroxy-3-methylglutaryl-CoA
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.8.3.13 succinylCoA:3-hydroxy-3-methylglutarate coenzyme A transferase
-
 Rattus norvegicus
2.8.3.13 succinylCoA:HMG coenzyme A transferase
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.13 8.5
-
-
 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.3.13 6
-
 decreasing the pH to lower values causes an irreversible loss of the enzyme activity Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.3.13 0.5
-
 acetoacetate pH 8.5, 30°C Rattus norvegicus
2.8.3.13 1.5
-
 succinate pH 8.5, 30°C Rattus norvegicus
2.8.3.13 2.1
-
 malonate pH 8.5, 30°C Rattus norvegicus