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Literature summary extracted from

  • Turnquist, R.L.; Gillett, T.A.; Hansen, R.G.
    Uridine diphosphate glucose pyrophosphorylase. Crystallization and properties of the enzyme from rabbit liver and species comparisons (1974), J. Biol. Chem., 249, 7695-7700.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.9 2-mercaptoethanol
-
 Bos taurus
2.7.7.9 2-mercaptoethanol requirement Homo sapiens
2.7.7.9 2-mercaptoethanol no activation Oryctolagus cuniculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.9 from ammonium sulfate precipitate Bos taurus
2.7.7.9 from ammonium sulfate precipitate Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.7.9 enzyme requires 2-mercaptoethanol for stability Homo sapiens
2.7.7.9 enzyme requires 2-mercaptoethanol for stability Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.9 alpha-D-galactose 1-phosphate
-
 Bos taurus
2.7.7.9 alpha-D-galactose 1-phosphate competitive Oryctolagus cuniculus
2.7.7.9 UDP-galactose
-
 Bos taurus
2.7.7.9 UDP-galactose
-
 Homo sapiens
2.7.7.9 UDP-galactose competitive Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.03 0.048 alpha-D-glucose 1-phosphate
-
 Bos taurus
2.7.7.9 0.03 0.048 alpha-D-glucose 1-phosphate pH 7.8 Oryctolagus cuniculus
2.7.7.9 0.048
-
 UTP pH 7.8 Oryctolagus cuniculus
2.7.7.9 0.048
-
 UTP liver Homo sapiens
2.7.7.9 0.05 0.066 UDP-glucose
-
 Homo sapiens
2.7.7.9 0.05 0.066 UDP-glucose
-
 Bos taurus
2.7.7.9 0.05 0.066 UDP-glucose pH 7.8 Oryctolagus cuniculus
2.7.7.9 0.055 0.08 alpha-D-glucose 1-phosphate
-
 Bos taurus
2.7.7.9 0.055 0.08 alpha-D-glucose 1-phosphate pH 7.8 Oryctolagus cuniculus
2.7.7.9 0.084
-
 diphosphate
-
 Bos taurus
2.7.7.9 0.084
-
 diphosphate pH 7.8 Oryctolagus cuniculus
2.7.7.9 0.095
-
 alpha-D-glucose 1-phosphate
-
 Homo sapiens
2.7.7.9 0.095
-
 alpha-D-glucose 1-phosphate pH 7.8 Oryctolagus cuniculus
2.7.7.9 0.21
-
 diphosphate
-
 Homo sapiens
2.7.7.9 0.21
-
 diphosphate pH 7.8 Oryctolagus cuniculus
2.7.7.9 0.42
-
 UDP-galactose pH 7.8 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Ca2+ activation Homo sapiens
2.7.7.9 Ca2+ activation Bos taurus
2.7.7.9 Ca2+ activation Oryctolagus cuniculus
2.7.7.9 Ca2+ 28% as effective as Mg2+ at 1 mM Bos taurus
2.7.7.9 Ca2+ 16% as effective as Mg2+ at 2 mM Homo sapiens
2.7.7.9 Ca2+ 50% as effective as Mg2+ at 1 mM Oryctolagus cuniculus
2.7.7.9 Co2+ activation Homo sapiens
2.7.7.9 Co2+ activation Bos taurus
2.7.7.9 Co2+ activation Oryctolagus cuniculus
2.7.7.9 Co2+ 75% activity at 2 mM Bos taurus
2.7.7.9 Co2+ 47-49% activity at 2 mM Homo sapiens
2.7.7.9 Co2+ 47-49% activity at 2 mM Oryctolagus cuniculus
2.7.7.9 Mg2+ requirement Homo sapiens
2.7.7.9 Mg2+ requirement Bos taurus
2.7.7.9 Mg2+ requirement Oryctolagus cuniculus
2.7.7.9 Mn2+
-
 Homo sapiens
2.7.7.9 Mn2+ maximal activity at 1 mM Bos taurus
2.7.7.9 Mn2+ maximal activity at 1 mM Oryctolagus cuniculus
2.7.7.9 additional information requirement for divalent cations Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 50000
-
 8 * 50000, SDS-PAGE, stacked tetrameric configuration Oryctolagus cuniculus
2.7.7.9 56000
-
 8 * 56000, SDS-PAGE Homo sapiens
2.7.7.9 60000
-
 8 * 60000, SDS-PAGE Bos taurus
2.7.7.9 400000
-
 sucrose density gradient centrifugation Oryctolagus cuniculus
2.7.7.9 450000
-
 sucrose density gradient centrifugation Homo sapiens
2.7.7.9 480000
-
 sucrose density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Bos taurus
-
 crystallized liver enzyme
-
2.7.7.9 Homo sapiens
-
 crystallized liver enzyme
-
2.7.7.9 Oryctolagus cuniculus
-
 crystallized liver enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 from liver Oryctolagus cuniculus
2.7.7.9 preparative sucrose density gradient centrifugation Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 liver
-
 Homo sapiens
-
2.7.7.9 liver
-
 Bos taurus
-
2.7.7.9 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 200
-
-
 Homo sapiens
2.7.7.9 200
-
-
 Bos taurus
2.7.7.9 200
-
 purified enzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 diphosphate + TDP-glucose poor substrate Homo sapiens TTP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.9 diphosphate + TDP-glucose poor substrate Bos taurus TTP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.9 diphosphate + TDP-glucose poor substrate Oryctolagus cuniculus TTP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.9 diphosphate + UDP-xylose poor substrate Homo sapiens UTP + D-xylose 1-phosphate
-
 r
2.7.7.9 diphosphate + UDP-xylose poor substrate Bos taurus UTP + D-xylose 1-phosphate
-
 r
2.7.7.9 diphosphate + UDP-xylose poor substrate Oryctolagus cuniculus UTP + D-xylose 1-phosphate
-
 r
2.7.7.9 UTP + alpha-D-galactose 1-phosphate about 15% of the activity with UDP-glucose Bos taurus diphosphate + UDP-galactose
-
 r
2.7.7.9 UTP + alpha-D-galactose 1-phosphate equilibrium dialysis: 8 mol UDP-galactose bound per mol enzyme Oryctolagus cuniculus diphosphate + UDP-galactose
-
 r
2.7.7.9 UTP + alpha-D-galactose 1-phosphate 3.5% of the activity with UDP-glucose Oryctolagus cuniculus diphosphate + UDP-galactose
-
 r
2.7.7.9 UTP + alpha-D-galactose 1-phosphate 1-12% of the activity with UDP-glucose Homo sapiens diphosphate + UDP-galactose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Homo sapiens diphosphate + UDP-glucose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Bos taurus diphosphate + UDP-glucose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate equilibrium dialysis: 8 mol UDP-glucose bound per mol enzyme Oryctolagus cuniculus diphosphate + UDP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.9 octamer 8 * 50000, SDS-PAGE, stacked tetrameric configuration Oryctolagus cuniculus
2.7.7.9 octamer 8 * 56000, SDS-PAGE Homo sapiens
2.7.7.9 octamer 8 * 60000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 7 9.5 broad Bos taurus
2.7.7.9 7 10.5 broad Oryctolagus cuniculus
2.7.7.9 7.6 9.2 broad Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.9 additional information
-
 additional information
-
 Homo sapiens
2.7.7.9 additional information
-
 additional information
-
 Bos taurus
2.7.7.9 additional information
-
 additional information
-
 Oryctolagus cuniculus