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Literature summary extracted from

  • Hunt, R.E.; Cowles, J.R.
    Physiological levels and properties of polynucleotide phosphorylase of Rhizobium meliloti (1977), J. Gen. Microbiol., 102, 403-411.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.8 AMP 1.6 mM, 30% activation of ADP polymerization Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.8 ATP 1.6 mM, 25% inhibition of ADP polymerization Sinorhizobium meliloti
2.7.7.8 dADP 0.16 mM, more than 80% inhibition of ADP polymerization Sinorhizobium meliloti
2.7.7.8 GDP 0.8 mM, more than 80% inhibition of ADP polymerization Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.8 0.32
-
 Poly(A) pH 8.0, 37°C Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.8 Mg2+ required for activity Sinorhizobium meliloti
2.7.7.8 Mg2+ optimal concentration for polymerization and phosphorolysis at 0.4 mM, optimum nucleotide/Mg2+ ratios for ADP, CDP and UDP are 4/1, 4/1 and 5/1, respectively Sinorhizobium meliloti
2.7.7.8 Mn2+ can partially replace Mg2+ in activation Sinorhizobium meliloti
2.7.7.8 Mn2+ effective polymer:Mg2+ ratio is 1:1 Sinorhizobium meliloti
2.7.7.8 Mn2+ 20-30% of activity with Mg2+ Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Bradyrhizobium japonicum
-
-
-
2.7.7.8 Sinorhizobium meliloti
-
-
-
2.7.7.8 Sinorhizobium meliloti F-28
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 heat, ammonium sulfate, Sephadex G-200, DEAE-cellulose Sinorhizobium meliloti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.8 bacteroid free-living and symbiotic Sinorhizobium meliloti
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 0.02
-
 ADP polymerization in crude extract Bradyrhizobium japonicum
2.7.7.8 3.8
-
 ADP polymerization Sinorhizobium meliloti

Storage Stability

EC Number Storage Stability Organism
2.7.7.8 4°C, at least 6 months, no loss of activity Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 poly(A) + ADP
-
 Sinorhizobium meliloti poly(A)+1 + phosphate
-
 r
2.7.7.8 poly(C) + CDP
-
 Sinorhizobium meliloti poly(C)+1 + phosphate
-
 r
2.7.7.8 poly(G) + GDP 10% of activity with ADP and poly(A) Sinorhizobium meliloti poly(G)+1 + phosphate less than 15% of phosphorolysis activity with poly(A) r
2.7.7.8 poly(U)+ UDP
-
 Sinorhizobium meliloti poly(U)+1 + phosphate
-
 r
2.7.7.8 ribonucleoside 5'-diphosphate + phosphate exchange reaction with ADP, CDP, UDP and GDP Sinorhizobium meliloti ribonucleoside 5'-diphosphate + phosphate exchange reaction ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of CDP Sinorhizobium meliloti RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of GDP Sinorhizobium meliloti RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate ADP best substrate, UDP 55%, CDP 51%, IDP 48% of activity with ADP Sinorhizobium meliloti RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of UDP Sinorhizobium meliloti RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Sinorhizobium meliloti RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of CDP Sinorhizobium meliloti F-28 RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of GDP Sinorhizobium meliloti F-28 RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate ADP best substrate, UDP 55%, CDP 51%, IDP 48% of activity with ADP Sinorhizobium meliloti F-28 RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of UDP Sinorhizobium meliloti F-28 RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Sinorhizobium meliloti F-28 RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn+1 + phosphate poly(A), poly(U) and poly(C) most effective substrates Sinorhizobium meliloti RNAn + a nucleoside diphosphate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 8
-
 phosphorolysis Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.8 AMP stimulates polymerization of ADP Sinorhizobium meliloti