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Literature summary extracted from

  • Soe, G.; Yamashita, J.
    Purification and properties of polynucleotide phosphorylase from photosynthetic bacterium Rhodospirillum rubrum (1980), J. Biochem., 87, 101-110.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.8 ADP inhibits ADP-phosphate exchange Rhodospirillum rubrum
2.7.7.8 phosphate inhibition of ADP-phosphate exchange Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.8 0.25
-
phosphate pH 8.0, 20°C, ADP-phosphate-exchange reaction Rhodospirillum rubrum
2.7.7.8 1
-
ADP pH 8.0, 20°C, ADP-phosphate exchange reaction Rhodospirillum rubrum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.8 Cd2+ stimulates Rhodospirillum rubrum
2.7.7.8 Co2+ stimulates Rhodospirillum rubrum
2.7.7.8 Mg2+ optimal concentration for polymerization, phosphorolysis and ADP-phosphate exchange at 1 mM, 1-3 mM and 3 mM, respectively Rhodospirillum rubrum
2.7.7.8 Mn2+ optimal concentration for polymerization, phosphorolysis and ADP-phosphate exchange at 1 mM Rhodospirillum rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.8 76000
-
2 * 76000, SDS-PAGE Rhodospirillum rubrum
2.7.7.8 160000
-
gel filtration Rhodospirillum rubrum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Rhodospirillum rubrum
-
photosynthetic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 DEAE-cellulose, ammonium sulfate, Sephadex G-200 Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 additional information
-
0.0006 mmol/absorbance at 280 nm/min, ADP-P exchange activity Rhodospirillum rubrum

Storage Stability

EC Number Storage Stability Organism
2.7.7.8 4°C Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 poly(A) + ADP
-
Rhodospirillum rubrum poly(A)+1 + phosphate 32% of activity with poly(U) in phosphorolysis reaction r
2.7.7.8 poly(A)+1 + phosphate
-
Rhodospirillum rubrum poly(A) + ADP
-
r
2.7.7.8 poly(C) + CDP 51% of activity with ADP Rhodospirillum rubrum poly(C)+1 + phosphate 21% of activity with poly(U) in phosphorolysis reaction r
2.7.7.8 poly(C)+1 + phosphate
-
Rhodospirillum rubrum poly(C) + ADP
-
r
2.7.7.8 poly(G) + GDP very little activity Rhodospirillum rubrum poly(G)+1 + phosphate
-
r
2.7.7.8 poly(I) + IDP 48% of activity with ADP Rhodospirillum rubrum poly(I)+1 + phosphate 28% of activity with poly(U) in phosphorolysis reaction r
2.7.7.8 poly(U)+ UDP 55% of activity with ADP Rhodospirillum rubrum poly(U)+1 + phosphate
-
r
2.7.7.8 poly(U)+ UDP best substrate for phosphorolysis reaction Rhodospirillum rubrum poly(U)+1 + phosphate
-
r
2.7.7.8 poly(U)+1 + phosphate
-
Rhodospirillum rubrum poly(U) + ADP
-
r
2.7.7.8 ribonucleoside 5'-diphosphate + phosphate ADP, GDP and CDP are better substrates than UDP, IDP and deoxribonucloside diphosphates do not serve as substrate Rhodospirillum rubrum ribonucleoside 5'-diphosphate + phosphate exchange reaction ?
2.7.7.8 RNAn + a nucleoside diphosphate ADP, GDP and CTP are better substrates than IDP and UDP Rhodospirillum rubrum RNAn+1 + phosphate
-
r
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of IDP Rhodospirillum rubrum RNAn+1 + phosphate
-
r
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of CDP Rhodospirillum rubrum RNAn+1 + phosphate
-
r
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of GDP Rhodospirillum rubrum RNAn+1 + phosphate
-
r
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of UDP Rhodospirillum rubrum RNAn+1 + phosphate
-
r
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Rhodospirillum rubrum RNAn+1 + phosphate
-
r
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(U) Rhodospirillum rubrum RNAn + a nucleoside diphosphate
-
r
2.7.7.8 RNAn+1 + phosphate poly(U) best substrate, yeast RNA 2%, poly(A) 32%, poly(I) 28%, poly(C) 21% of the activity with poly(U) Rhodospirillum rubrum RNAn + a nucleoside diphosphate
-
r
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(I) Rhodospirillum rubrum RNAn + a nucleoside diphosphate
-
r
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(A) Rhodospirillum rubrum RNAn + a nucleoside diphosphate
-
r
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(C) Rhodospirillum rubrum RNAn + a nucleoside diphosphate
-
r
2.7.7.8 yeast RNA+1 + phosphate 2% of activity with poly(U) Rhodospirillum rubrum yeast RNA + nucleoside diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.8 dimer 2 * 76000, SDS-PAGE Rhodospirillum rubrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 7.2
-
phosphorolysis Rhodospirillum rubrum
2.7.7.8 7.8
-
ADP-phosphate exchange reaction Rhodospirillum rubrum
2.7.7.8 10
-
or above, polymerization Rhodospirillum rubrum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.8 5 10 incubation at 4°C for 2 d, no loss of activity, rapid inactivation below pH 5.0 Rhodospirillum rubrum