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Literature summary extracted from

  • Godefroy-Colburn, T.; Grunenberg-Manago, M.
    Polynucleotide phosphorylase (1972), The Enzymes, 3rd. Ed. (Boyer, P. D. , ed. ), 7, 533-574.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.8 Poly(A) inhibition of oligadenylate and oligouridylate phosphorolysis Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.8 additional information
-
 additional information values for Km with polynucleotides longer than 20 nucleotides are much smaller than the Km for oligonucleotides which lies at approx. 0.05 mM Escherichia coli
2.7.7.8 0.033
-
 nonaadenylate phosphorolysis reaction Micrococcus luteus
2.7.7.8 0.05
-
 heptauridylate phosphorolysis reaction Micrococcus luteus
2.7.7.8 0.25
-
 tetrauridylate phosphorolysis reaction Micrococcus luteus
2.7.7.8 2.5
-
 triadenylate phosphorolysis reaction Micrococcus luteus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.8 cell membrane
-
 Halobacterium salinarum
-
-
2.7.7.8 chloroplast
-
 Spinacia oleracea 9507
-
2.7.7.8 chloroplast
-
 Auxenochlorella pyrenoidosa 9507
-
2.7.7.8 nucleus
-
 Rattus norvegicus 5634
-
2.7.7.8 nucleus HeLa cells Homo sapiens 5634
-
2.7.7.8 ribosome
-
 Synechococcus elongatus PCC 7942 = FACHB-805 5840
-
2.7.7.8 soluble
-
 Escherichia coli
-
-
2.7.7.8 soluble
-
 Pseudomonas aeruginosa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.8 RNAn + a nucleoside diphosphate Spinacia oleracea
-
 RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate Escherichia coli PNPase is involved in RNA degradation RNAn+1 + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Ascaris lumbricoides
-
-
-
2.7.7.8 Auxenochlorella pyrenoidosa
-
-
-
2.7.7.8 Cavia porcellus
-
-
-
2.7.7.8 Escherichia coli
-
-
-
2.7.7.8 Halobacterium salinarum
-
-
-
2.7.7.8 Homo sapiens
-
-
-
2.7.7.8 Lactiplantibacillus plantarum
-
-
-
2.7.7.8 Micrococcus luteus
-
-
-
2.7.7.8 Neisseria meningitidis
-
-
-
2.7.7.8 Pseudomonas aeruginosa
-
-
-
2.7.7.8 Rattus norvegicus
-
-
-
2.7.7.8 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.7.7.8 Spinacia oleracea
-
-
-
2.7.7.8 Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-
2.7.7.8 Triticum aestivum
-
 wheat
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Salmonella enterica subsp. enterica serovar Typhimurium
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Synechococcus elongatus PCC 7942 = FACHB-805
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Cavia porcellus
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Lactiplantibacillus plantarum
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Escherichia coli
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Homo sapiens
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Rattus norvegicus
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Spinacia oleracea
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Pseudomonas aeruginosa
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Halobacterium salinarum
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Neisseria meningitidis
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Auxenochlorella pyrenoidosa
a
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate mechanism of elongation, overview Ascaris lumbricoides
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.8 brain
-
 Rattus norvegicus
-
2.7.7.8 epithelioma cell
-
 Rattus norvegicus
-
2.7.7.8 leaf
-
 Spinacia oleracea
-
2.7.7.8 liver
-
 Cavia porcellus
-
2.7.7.8 liver
-
 Rattus norvegicus
-
2.7.7.8 root
-
 Triticum aestivum
-
2.7.7.8 sperm
-
 Homo sapiens
-
2.7.7.8 urine
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 RNAn + a nucleoside diphosphate
-
 Spinacia oleracea RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Salmonella enterica subsp. enterica serovar Typhimurium RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Synechococcus elongatus PCC 7942 = FACHB-805 RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Cavia porcellus RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Lactiplantibacillus plantarum RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Escherichia coli RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Homo sapiens RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Spinacia oleracea RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Pseudomonas aeruginosa RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Halobacterium salinarum RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Neisseria meningitidis RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Micrococcus luteus RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Auxenochlorella pyrenoidosa RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate specificity overview Ascaris lumbricoides RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate primer-dependent activity Triticum aestivum RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate PNPase is involved in RNA degradation Escherichia coli RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Synechococcus elongatus PCC 7942 = FACHB-805 RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Cavia porcellus RNAn + a nucleoside diphosphate
-
 ?
2.7.7.8 RNAn+1 + phosphate
-
 Lactiplantibacillus plantarum RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Escherichia coli RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Homo sapiens RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Rattus norvegicus RNAn + a nucleoside diphosphate
-
 ?
2.7.7.8 RNAn+1 + phosphate
-
 Spinacia oleracea RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Pseudomonas aeruginosa RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Halobacterium salinarum RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Neisseria meningitidis RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Micrococcus luteus RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Auxenochlorella pyrenoidosa RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate
-
 Ascaris lumbricoides RNAn + a nucleoside diphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.8 36
-
 synthesis of poly U Micrococcus luteus
2.7.7.8 45 55 polymerization of UDP and CDP Escherichia coli
2.7.7.8 60
-
 polymerization of ADP and GDP, phosphorolysis of poly A Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.8 36
-
-
 Micrococcus luteus
2.7.7.8 55 60 10 min, no loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 8 9.5
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.8 8 9.5
-
 Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.8 8 9.5
-
 Cavia porcellus
2.7.7.8 8 9.5
-
 Lactiplantibacillus plantarum
2.7.7.8 8 9.5
-
 Escherichia coli
2.7.7.8 8 9.5
-
 Homo sapiens
2.7.7.8 8 9.5
-
 Rattus norvegicus
2.7.7.8 8 9.5
-
 Spinacia oleracea
2.7.7.8 8 9.5
-
 Pseudomonas aeruginosa
2.7.7.8 8 9.5
-
 Halobacterium salinarum
2.7.7.8 8 9.5
-
 Neisseria meningitidis
2.7.7.8 8 9.5
-
 Auxenochlorella pyrenoidosa
2.7.7.8 8 9.5
-
 Ascaris lumbricoides