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Literature summary extracted from

  • Palm, P.; Schleper, C.; Arnold-Ammer, I.; Holz, I.; Meier, T.; Lottspeich, F.; Zillig, W.
    The DNA-dependent RNA-polymerase of Thermotoga maritima; characterisation of the enzyme and the DNA-sequence of the genes for the large subunits (1993), Nucleic Acids Res., 21, 4904-4908.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6 the genes for the large subunits beta and beta‘ are cloned and sequenced. MW for beta‘ and beta, calculated from nucleotide sequence is 190522 Da and 143139 Da Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 45000
-
 beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima
2.7.7.6 55000
-
 beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima
2.7.7.6 141000
-
 beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima
2.7.7.6 184000
-
 beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Thermotoga maritima diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme is highly active with poly dAT or T7 phage DNA as template Thermotoga maritima diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 ? beta‘,beta,sigma,alpha occur with a stoichiometric ratio of 1:1:1:2, x * 184000 + x * 141000 + x * 55000 + x * 45000, SDS-PAGE Thermotoga maritima