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Literature summary extracted from
- Purification and characterization of DNA dependent RNA polymerase from Staphylococcus aureus (1995), Biochem. Biophys. Res. Commun., 208, 610-616.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.7.6 | KCl | maximal activity at 200 mM | Staphylococcus aureus |  |
| 2.7.7.6 | Mg2+ | maximal activity at 10-30 mM | Staphylococcus aureus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.6 | 42000 | - |
x * 151000 + x * 147000 + x * 55000 + x * 42000, SDS-PAGE | Staphylococcus aureus |
| 2.7.7.6 | 55000 | - |
x * 151000 + x * 147000 + x * 55000 + x * 42000, SDS-PAGE | Staphylococcus aureus |
| 2.7.7.6 | 147000 | - |
x * 151000 + x * 147000 + x * 55000 + x * 42000, SDS-PAGE | Staphylococcus aureus |
| 2.7.7.6 | 151000 | - |
x * 151000 + x * 147000 + x * 55000 + x * 42000, SDS-PAGE | Staphylococcus aureus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.7.6 | Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.7.6 | - |
Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.6 | 0.0813 | - |
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.7.6 | nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Staphylococcus aureus | diphosphate + RNAn+1 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.7.6 | ? | x * 151000 + x * 147000 + x * 55000 + x * 42000, SDS-PAGE | Staphylococcus aureus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.6 | 40 | 45 | - |
Staphylococcus aureus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.7.6 | 37 | 50 | 37°C: about 25% of maximal activity, 50°C: about 5% of maximal activity | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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