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Literature summary extracted from

  • Schiebel, W.; Haas, B.; Marinkovic, S.; Klanner, A.; Sไnger, H.L.
    RNA-directed RNA polymerase from tomato leaves. II. Catalytic in vitro properties (1993), J. Biol. Chem., 268, 11858-11867.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Solanum lycopersicum
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 inoculated with potato spindle tuber viroid. In viroid-infected tomato leaf the activity of the host-encoded RdRP is significantly increased. Viroids are not translated into proteins so that they cannot code for a viroid-specific RNA replicase
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.48 leaf leaf inoculated with potato spindle tuber viroid. In viroid-infected tomato leaf the activity of the host-encoded RdRP is significantly increased. Viroids are not translated into proteins so that they cannot code for a viroid-specific RNA replicase Solanum lycopersicum
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn the enzyme catalyzes in vitro the transcription of short single-stranded RNA and DNA molecules into precise complementary RNA copies up to the full length of these templates. The transcription of RNA-oligonicleotide templates and DNA-oligonucleotide templates is equally effective. Differences in transcription efficiency are found to depend on nucleotide sequence rather than on the RNA or DNA nature of the single-stranded nucleic acid. Double-stranded nucleic acids such as poly(A)*poly(U) and a double-stranded DNA 14-mer are not transcribed. The RdRP-directed transcription can be primed. The unprimed transcription starts preferentially at the 3'-terminal nucleotide of the template. The enzyme is capable of adding a single noncomplementary nucleotide to the 3'-terminus of about 50% of the runoff transcripts. AMP is preferred over GMP whereas CMP and UMP are terminally added at very low frequency Solanum lycopersicum diphosphate + RNAn+1
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