Literature summary extracted from

Heacock, A.M.; Agranoff, B.W.
CDP-diacylglycerol synthase from mammalian tissues (1997), Biochim. Biophys. Acta, 1348, 166-172.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.7.41	-	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.7.7.41	1	-	CTP	-	Cavia porcellus
2.7.7.41	2.5	-	phosphatidate	-	Cavia porcellus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.7.7.41	microsome	predominantly located in	Rattus norvegicus	-	-
2.7.7.41	mitochondrion	5-10% of the cellular activity	Rattus norvegicus	5739	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism
2.7.7.41	Mg2+	-	Escherichia coli
2.7.7.41	Mg2+	-	Sus scrofa
2.7.7.41	Mg2+	50-60 mM required for optimal activity	Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
2.7.7.41	CTP + phosphatidate	Cavia porcellus	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	Escherichia coli	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	Homo sapiens	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	Rattus norvegicus	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	Sus scrofa	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	Rattus norvegicus	enzyme plays a central role in phospholipid biosynthesis	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	Drosophila sp. (in: flies)	the enzyme plays a regulatory role in phototransduction by ensuring an adequate supply of phosphatidylinositol-4,5-diphosphate	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	Cavia porcellus	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	Escherichia coli	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	Homo sapiens	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	Rattus norvegicus	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	Sus scrofa	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	diphosphate + dCDPdiacylglycerol	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.41	Cavia porcellus	-	-	-
2.7.7.41	Drosophila sp. (in: flies)	-	-	-
2.7.7.41	Escherichia coli	-	-	-
2.7.7.41	Homo sapiens	-	-	-
2.7.7.41	Rattus norvegicus	-	-	-
2.7.7.41	Sus scrofa	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.7.7.41	brain	-	Rattus norvegicus	-
2.7.7.41	eye	-	Drosophila sp. (in: flies)	-
2.7.7.41	liver	-	Cavia porcellus	-
2.7.7.41	liver	-	Rattus norvegicus	-
2.7.7.41	neuronal cell line	-	Homo sapiens	-
2.7.7.41	skeletal muscle	low activity	Rattus norvegicus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2.7.7.41	CTP + phosphatidate	-	Cavia porcellus	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	-	Drosophila sp. (in: flies)	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	-	Escherichia coli	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	-	Homo sapiens	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	-	Rattus norvegicus	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	-	Sus scrofa	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Cavia porcellus	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Escherichia coli	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Homo sapiens	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Rattus norvegicus	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Sus scrofa	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	enzyme plays a central role in phospholipid biosynthesis	Rattus norvegicus	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	CTP + phosphatidate	the enzyme plays a regulatory role in phototransduction by ensuring an adequate supply of phosphatidylinositol-4,5-diphosphate	Drosophila sp. (in: flies)	diphosphate + CDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	-	Cavia porcellus	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	-	Homo sapiens	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	-	Rattus norvegicus	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	-	Sus scrofa	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	reaction at the same rate as CTP	Escherichia coli	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Cavia porcellus	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Escherichia coli	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Homo sapiens	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Rattus norvegicus	diphosphate + dCDPdiacylglycerol	-	?
2.7.7.41	dCTP + phosphatidate	mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme	Sus scrofa	diphosphate + dCDPdiacylglycerol	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.7.7.41	6.8	-	-	Rattus norvegicus