Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Langley, K.E.; Kennedy, E.P.
    Partial purification and properties of CTP:phosphatidic acid cytidylyltransferase from membranes of Escherichia coli (1978), J. Bacteriol., 136, 85-95.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.41 ATP a maximal stimulation of about 3fold with CTP as substrate and 1.25fold with dCTP Escherichia coli

General Stability

EC Number General Stability Organism
2.7.7.41 solubilized enzyme is stable to freezing and thawing Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.41 CDP-dipalmitin inhibits diphosphorolysis of CDP-dipalmitin Escherichia coli
2.7.7.41 CTP competitive with dCTP Escherichia coli
2.7.7.41 dCTP competitive with respect to CTP Escherichia coli
2.7.7.41 EDTA
-
 Escherichia coli
2.7.7.41 SDS 0.1% w/v Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.41 0.28
-
 phosphatidic acid pH 7, 37°C Escherichia coli
2.7.7.41 0.9
-
 dCTP pH 7, 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.41 cell envelope
-
 Escherichia coli 30313
-
2.7.7.41 membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.41 CTP + phosphatidate Escherichia coli the enzyme produces key intermediates in phospholipid biosynthesis diphosphate + CDPdiacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.41 partial Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.41 CTP + phosphatidate = diphosphate + CDP-diacylglycerol sequential mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.41 0.028
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.41 -70°C, solubilized enzyme is stable for at least several months Escherichia coli
2.7.7.41 0-4°C, solubilized enzyme is stable for at least several weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + phosphatidate
-
 Escherichia coli diphosphate + CDPdiacylglycerol
-
 r
2.7.7.41 CTP + phosphatidate the enzyme produces key intermediates in phospholipid biosynthesis Escherichia coli diphosphate + CDPdiacylglycerol
-
 ?
2.7.7.41 dCTP + phosphatidate
-
 Escherichia coli diphosphate + dCDPdiacylglycerol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.41 57
-
 30 min, no loss of activity of membrane-bound enzyme, 70% loss of activity of partially purified enzyme Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.41 7
-
 stability is good at pH 7 and dropps rapidly above Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.41 0.48
-
 CTP pH 7, 37°C Escherichia coli
2.7.7.41 0.75
-
 dCTP pH 7, 37°C Escherichia coli