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Literature summary extracted from

  • Carman, G.M.; Kelley, M.J.
    CDPdiacylglycerol synthase from yeast (1992), Methods Enzymol., 209, 242-247.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.41 stable to at least 2 cycles of freezing and thawing Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.41 dCTP competitive with respect to CTP Saccharomyces cerevisiae
2.7.7.41 Thiophosphatidate competitive Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.41 0.5
-
 phosphatidate
-
 Saccharomyces cerevisiae
2.7.7.41 1
-
 CTP pH 6.5, 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.41 microsome about 20% of enzyme is associated with Saccharomyces cerevisiae
-
-
2.7.7.41 mitochondrion main site of localization Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.41 114000
-
-
 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.41 mitochondrial enzyme Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.41 CTP + phosphatidate = diphosphate + CDP-diacylglycerol sequential bi-bi reaction: CDPdiacylglycerol synthase binds to CTP prior to phosphatidate and diphosphate is released prior to CDPdiacylglycerol in the reaction sequence Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.41 additional information
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.7.41 -80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + phosphatidate
-
 Saccharomyces cerevisiae diphosphate + CDPdiacylglycerol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.41 30
-
 20 min, labile above Saccharomyces cerevisiae