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Literature summary extracted from

  • Evans, W.R.; Pietro, A.S.
    Phosphorolysis of adenosine diphosphoribose (1966), Arch. Biochem. Biophys., 113, 236-244.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.35 ADP-ribose competitive to phosphate Euglena gracilis
2.7.7.35 Ag2+
-
 Euglena gracilis
2.7.7.35 D-ribose 5-phosphate competitive to phosphate with ADP, noncompetitive to phosphate with ADP-ribose Euglena gracilis
2.7.7.35 deoxyribose 5-phosphate ADP/phosphate-exchange Euglena gracilis
2.7.7.35 additional information no inhibition by CN-, 1,10-phenanthroline, 2,2'-dipyridyl, ribose 1-phosphate, ribose 1,5-diphosphate, glucose 1-phosphate, glucose 6-phosphate; no inhibition by NADP+, EDTA, ribose Euglena gracilis
2.7.7.35 PCMB ADP/phosphate-exchange, reversible by cysteine Euglena gracilis
2.7.7.35 SeO42-
-
 Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.35 additional information
-
 additional information kinetic study Euglena gracilis
2.7.7.35 0.04
-
 ADP-ribose pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.4
-
 phosphate pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.6
-
 ADP pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.6
-
 ADP ADP/phosphate exchange Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.35 cytosol
-
 Euglena gracilis 5829
-
2.7.7.35 cytosol
-
 Ochromonas danica 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.35 additional information no metal ion requirement Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.35 phosphate + ADP-ribose Euglena gracilis
-
 ADP + D-ribose 5-phosphate
-
 ir
2.7.7.35 phosphate + ADP-ribose Ochromonas danica
-
 ADP + D-ribose 5-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.35 Euglena gracilis
-
 green alga, strain Z
-
2.7.7.35 no activity in Chlorella sp.
-
-
-
2.7.7.35 no activity in Gloeocapsa sp.
-
-
-
2.7.7.35 no activity in Pisum sativum
-
-
-
2.7.7.35 no activity in Spinacia oleracea
-
-
-
2.7.7.35 Ochromonas danica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.35 partial Euglena gracilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.35 ADP + D-ribose 5-phosphate = phosphate + ADP-D-ribose ping-pong reaction mechanism Euglena gracilis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.35 cell culture
-
 Ochromonas danica
-
2.7.7.35 cell culture heterotrophically or autotrophically grown cells Euglena gracilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.35 additional information
-
 low activity Ochromonas danica
2.7.7.35 1.3
-
 partially purified enzyme Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
2.7.7.35 frozen, fairly stable in the presence of EDTA Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.35 additional information no activity with CDP, GDP, UDP, ADP-glucose and UDP-glucose Euglena gracilis ?
-
 ?
2.7.7.35 additional information no activity with crude polyadenylic acid, RNA from Euglena, and NAD(H), NADP(H), coenzyme A, FAD Euglena gracilis ?
-
 ?
2.7.7.35 phosphate + ADP IDP can replace ADP with 16% efficiency Euglena gracilis ADP + phosphate
-
 r
2.7.7.35 phosphate + ADP IDP can replace ADP with 16% efficiency Ochromonas danica ADP + phosphate
-
 r
2.7.7.35 phosphate + ADP-ribose
-
 Euglena gracilis ADP + D-ribose 5-phosphate
-
 ir
2.7.7.35 phosphate + ADP-ribose
-
 Ochromonas danica ADP + D-ribose 5-phosphate
-
 ?
2.7.7.35 phosphate + ADP-ribose
-
 Ochromonas danica ADP + D-ribose 5-phosphate
-
 ir
2.7.7.35 phosphate + ADP-ribose highly specific Euglena gracilis ADP + D-ribose 5-phosphate
-
 ir
2.7.7.35 phosphate + deamino-NAD+ inosine diphosphoribose, not adenosine triphosphoribose Euglena gracilis ?
-
 ?
2.7.7.35 phosphate + deamino-NAD+ inosine diphosphoribose, not adenosine triphosphoribose Ochromonas danica ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.35 7.5 7.8 ADP/phosphate-exchange Euglena gracilis
2.7.7.35 8
-
 ADP-ribose phosphorolysis Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.35 6.4 8.1 ADP/phosphate-exchange, about half-maximal activity at pH 6.4 and pH 8.1 Euglena gracilis
2.7.7.35 6.8 9 ADP-ribose phosphorolysis, about half-maximal activity at pH 6.8 and about 90% of maximal activity at pH 9.0 Euglena gracilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.35 0.11
-
 D-ribose 5-phosphate pH 7.8, 30°C Euglena gracilis
2.7.7.35 0.11
-
 D-ribose 5-phosphate ADP/phosphate-exchange reaction Euglena gracilis
2.7.7.35 4
-
 D-ribose 5-phosphate phosphorolysis, pH 7.8, 30°C Euglena gracilis