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Literature summary extracted from

  • Johnson, P.E.; Patron, N.J.; Bottrill, A.R.; Dinges, J.R.; Fahy, B.F.; Parker, M.L.; Waite, D.N.; Denyer, K.
    A low-starch barley mutant, riso 16, lacking the cytosolic small subunit of ADP-glucose pyrophosphorylase, reveals the importance of the cytosolic isoform and the identity of the plastidial small subunit (2003), Plant Physiol., 131, 684-696.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 Hv.AGP.S.1a. and Hv.AGP.S.1b. endcode small subunit cytosolic and plastidial proteins, but not major plastidial enzyme, Hv.AGP.S.2 endcodes the novel small subunit of the plastidial form of enzyme Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 additional information
-
 Hordeum vulgare
2.7.7.27 additional information in mutant Riso 16, the Hv.AGP.S.1. gene is substantially deleted and therefore inactive and lacks the cytosolic small subunit of enzyme in the endosperm and cytosolic enzyme activity, the Hv.AGP.S.2. gene is not affected in Riso and it has a normal plastidial enzyme activity Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Hordeum vulgare P55238
-
-
2.7.7.27 Hordeum vulgare P55238 barley 1a, cytosolic small subunit enzyme
-
2.7.7.27 Hordeum vulgare P55238 barley 1b, plastidial small subunit enzyme
-
2.7.7.27 Hordeum vulgare Q8HS72 plastidial enzyme
-
2.7.7.27 Oryza sativa Q9ARH9
-
-
2.7.7.27 Triticum aestivum P30523
-
-
2.7.7.27 Zea mays Q941P2
-
-
2.7.7.27 Zea mays Q947B9
-
-
2.7.7.27 Zea mays Q947C0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 cytosolic and plastidial enzyme of endosperm Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 endosperm
-
 Hordeum vulgare
-
2.7.7.27 endosperm
-
 Triticum aestivum
-
2.7.7.27 endosperm
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 11.6
-
 plastidial enzyme Hordeum vulgare
2.7.7.27 12.3
-
 cytosolic enzyme Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Oryza sativa diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Hordeum vulgare diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Triticum aestivum diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 ?