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Literature summary extracted from

  • Salamone, P.R.; Greene, T.W.; Kavakli, I.H.; Okita, T.W.
    Isolation and characterization of a higher plant ADP-glucose pyrophosphorylase small subunit homotetramer (2000), FEBS Lett., 482, 113-118.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate
-
 Zea mays
2.7.7.27 3-phosphoglycerate
-
 Solanum tuberosum
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Zea mays
2.7.7.27 D-fructose 1,6-bisphosphate
-
 Solanum tuberosum
2.7.7.27 D-fructose 6-phosphate
-
 Zea mays
2.7.7.27 D-fructose 6-phosphate
-
 Solanum tuberosum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 wild-type gene pML10 is mutated by hydroxylamine, the mutated SS gene TG-15 is expressed in Escherichia coli AC70R1-504 Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 L48F/V59I TG-15, significant alteration in effector sensitivity of this homotetrameric enzyme in comparison to wild-type heterotetrameric enzyme Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate
-
 Solanum tuberosum
2.7.7.27 phosphate
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 additional information
-
 additional information
-
 Zea mays
2.7.7.27 additional information
-
 additional information
-
 Solanum tuberosum
2.7.7.27 0.25
-
 alpha-D-glucose 1-phosphate pH 7.5, 37°C, mutant TG-15, kinetic study Solanum tuberosum
2.7.7.27 1.2
-
 ATP pH 7.5, 37°C, mutant TG-15, kinetic study Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+
-
 Solanum tuberosum
2.7.7.27 Mg2+ requirement Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 additional information
-
 amino acid composition and comparison Zea mays
2.7.7.27 additional information
-
 amino acid composition and comparison Synechocystis sp.
2.7.7.27 additional information
-
 amino acid composition and comparison Solanum tuberosum
2.7.7.27 additional information
-
 amino acid composition and comparison Anabaena sp.
2.7.7.27 additional information
-
 amino acid composition and comparison Escherichia coli
2.7.7.27 50000
-
 4 * 50000, the small subunit enzyme TG-15, SDS-PAGE Solanum tuberosum
2.7.7.27 200000
-
-
 Zea mays
2.7.7.27 200000
-
 gel filtration of homotetramer small subunit enzyme TG-15 Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Anabaena sp. P30521
-
-
2.7.7.27 Escherichia coli P0A6V1 glgC
-
2.7.7.27 Solanum tuberosum P23509 SS
-
2.7.7.27 Solanum tuberosum SS P23509 SS
-
2.7.7.27 Synechocystis sp. P52415
-
-
2.7.7.27 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 partial, homotetrameric small subunit enzyme TG-15, heat shock step at 55°C Solanum tuberosum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.27 ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-alpha-D-glucose the small and large subunits of the enzyme define the catalytic and regulatory subunits Solanum tuberosum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 additional information
-
-
 Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Synechocystis sp. diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Anabaena sp. diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Escherichia coli diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum SS diphosphate + ADP-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.27 homotetramer
-
 Zea mays
2.7.7.27 homotetramer 4 * 50000, the small subunit enzyme TG-15, SDS-PAGE Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 55
-
 purification of small subunit enzyme TG-15 involves a 7 min/55°C heat shock step Solanum tuberosum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.27 additional information
-
 additional information comparison of Ki of phosphate Solanum tuberosum