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Literature summary extracted from

  • Greene, T.W.; Hannah, L.C.
    Enhanced stability of maize endosperm ADP-glucose pyrophosphorylase is gained through mutants that alter subunit interactions (1998), Proc. Natl. Acad. Sci. USA, 95, 13342-13347.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 expression of the wild-type and mutant enzymes in Escherichia coli, Shrunken2, Sh2 encodes the large subunit and Brittle2, Bt2 encodes the small subunit, cloning and expression of the heat-stable variant Sh2hs33 and Bt2 in the yeast two-hybrid expression system Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 H333T Sh2hs33, coexpression with wild-type Brittle2: 5 min 60°C heat treatment, 76% remaining activity in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2, enhanced subunit interaction in this mutant Zea mays
2.7.7.27 H333T/T460I Sh2hs40, coexpression with wild-type Brittle2: 5 min 60°C heat treatment, 72% remaining activity in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2 Zea mays
2.7.7.27 R104T Sh2hs16, coexpression with wild-type Brittle2: 5 min 60°C heat treatment, 37% remaining activity in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2 Zea mays
2.7.7.27 R217P/H333T Sh2hs47, coexpression with wild-type Brittle2: 5min 60°C heat treatment, about 65% remaining activity in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 additional information
-
-
 Zea mays
2.7.7.27 200000
-
 density gradient centrifugation Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate Zea mays key regulatory enzyme of starch biosynthesis diphosphate + ADP-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 endosperm
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate key regulatory enzyme of starch biosynthesis Zea mays diphosphate + ADP-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.27 60
-
 76% remaining activity of mutant enzyme Sh2hs33 in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2 Zea mays