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Literature summary extracted from

  • Sowokinos, J.R.; Preiss, J.
    Pyrophphosphorylases in Solanum tuberosum, III. Purification, physical, and catalytic properties of ADPglucose pyrophophorylase in potatoes (1982), Plant Physiol., 69, 1459-1466.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate allosteric activation Solanum tuberosum
2.7.7.27 3-phosphoglycerate activation Solanum tuberosum
2.7.7.27 alpha-glycerol phosphate activation, pyrophosphorolysis, not Solanum tuberosum
2.7.7.27 D-fructose 1,6-bisphosphate slight activation Solanum tuberosum
2.7.7.27 D-fructose 1,6-bisphosphate activation of ADP-glucose synthesis Solanum tuberosum
2.7.7.27 D-ribose 5-phosphate activation Solanum tuberosum
2.7.7.27 D-ribose 5-phosphate activation of ADP-glucose synthesis Solanum tuberosum
2.7.7.27 DTT activation Solanum tuberosum
2.7.7.27 additional information not: L-lactate, citrate Solanum tuberosum
2.7.7.27 additional information not: oxaloacetate, L-malate Solanum tuberosum
2.7.7.27 additional information not: 2-oxoglutarate Solanum tuberosum
2.7.7.27 phosphoenolpyruvate activation Solanum tuberosum
2.7.7.27 phosphoenolpyruvate activation of pyrophosphorolysis Solanum tuberosum
2.7.7.27 phosphoenolpyruvate ADPglucose synthesis Solanum tuberosum
2.7.7.27 pyruvate not Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ADP less effective than phosphate Solanum tuberosum
2.7.7.27 AMP less effective than phosphate Solanum tuberosum
2.7.7.27 cyclic-3',5'-AMP less effective than phosphate Solanum tuberosum
2.7.7.27 NADP+ less effective than phosphate Solanum tuberosum
2.7.7.27 phosphate kinetics; strong Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.14
-
 alpha-D-glucose 1-phosphate pH 8.0, 37°C Solanum tuberosum
2.7.7.27 0.19
-
 ATP pH 8.0, 37°C Solanum tuberosum
2.7.7.27 0.24
-
 ADP-glucose pH 8.0, 37°C Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+ requirement Solanum tuberosum
2.7.7.27 Mg2+ ADPglucose synthesis Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.27 50000
-
 4 * 50000, SDS-PAGE Solanum tuberosum
2.7.7.27 200000
-
 sucrose density gradient centrifugation Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Solanum tuberosum
-
 potato, var. Norchip
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 partial Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 tuber growing Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.27 2.8
-
-
 Solanum tuberosum

Storage Stability

EC Number Storage Stability Organism
2.7.7.27 4°C, in 50 mM HEPES buffer, pH 7.5, 20% sucrose, 1 mM EDTA, 2 mM GSH, at least 6 weeks Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.27 homotetramer 4 * 50000, SDS-PAGE Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.27 8
-
 HEPES, bicine and glycylglycine buffer Solanum tuberosum
2.7.7.27 8
-
 ADP-glucose synthesis Solanum tuberosum
2.7.7.27 8.5
-
 Tris buffer Solanum tuberosum
2.7.7.27 8.5
-
 ADP-glucose synthesis Solanum tuberosum