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Literature summary extracted from

  • Sarkar, B.; Cao, G.J.; Sarkar, N.
    Identification of two poly(A) polymerases in Bacillus subtilis (1997), Biochem. Mol. Biol. Int., 41, 1045-1050.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.19 KCl major poly(A) polymerase, optimal at 250 mM, minor poly(A) polymerase, optimal at 125 mM, inhibition of both above 250 mM Bacillus subtilis
2.7.7.19 spermidine slight stimulation, 5 mM Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.19 1,10-phenanthroline 1 mM, 28% inhibition, major poly(A) polymerase, 50% inhibition, minor poly(A) polymerase Bacillus subtilis
2.7.7.19 cordycepin not Bacillus subtilis
2.7.7.19 CTP
-
Bacillus subtilis
2.7.7.19 dATP 0.25 mM, 50% inhibition, major poly(A) polymerase, 15% inhibition, minor poly(A) polymerase Bacillus subtilis
2.7.7.19 GTP
-
Bacillus subtilis
2.7.7.19 KCl stimulation at low concentrations, at 300 mM, 15% inhibition, major poly(A) polymerase, 52% inhibition, minor poly(A) polymerase Bacillus subtilis
2.7.7.19 additional information not inhibitory: rifampicin, streptolydigin, phosphate at 0.5 mM, cordycepin at 0.1 mM Bacillus subtilis
2.7.7.19 PO43- not inhibitory Bacillus subtilis
2.7.7.19 rifampicin not inhibitory Bacillus subtilis
2.7.7.19 UTP
-
Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 0.143
-
ATP major poly(A) polymerase, 37°C, pH 8.0 Bacillus subtilis
2.7.7.19 0.4
-
ATP major poly(A) polymerase, 37°C, pH 8.0 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.19 soluble
-
Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 Mg2+ more active in presence of Mn2+ than Mg2+ Bacillus subtilis
2.7.7.19 Mn2+ more active in presence of Mn2+ than Mg2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.19 37000
-
gel filtration, minor poly(A) polymerase Bacillus subtilis
2.7.7.19 86000
-
gel filtration, major poly(A) polymerase Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Bacillus subtilis
-
two isoforms
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA primer required Bacillus subtilis diphosphate + RNA(A)n
-
?
2.7.7.19 ATP + RNA enzyme also has cleavage activity Bacillus subtilis diphosphate + RNA(A)n
-
?
2.7.7.19 ATP + RNA primer: poly(A) Bacillus subtilis diphosphate + RNA(A)n
-
?
2.7.7.19 ATP + RNA primer: dephosphorylated poly(A), tRNA Bacillus subtilis diphosphate + RNA(A)n
-
?