EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.19 | KCl | major poly(A) polymerase, optimal at 250 mM, minor poly(A) polymerase, optimal at 125 mM, inhibition of both above 250 mM | Bacillus subtilis | |
2.7.7.19 | spermidine | slight stimulation, 5 mM | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.19 | 1,10-phenanthroline | 1 mM, 28% inhibition, major poly(A) polymerase, 50% inhibition, minor poly(A) polymerase | Bacillus subtilis | |
2.7.7.19 | cordycepin | not | Bacillus subtilis | |
2.7.7.19 | CTP | - |
Bacillus subtilis | |
2.7.7.19 | dATP | 0.25 mM, 50% inhibition, major poly(A) polymerase, 15% inhibition, minor poly(A) polymerase | Bacillus subtilis | |
2.7.7.19 | GTP | - |
Bacillus subtilis | |
2.7.7.19 | KCl | stimulation at low concentrations, at 300 mM, 15% inhibition, major poly(A) polymerase, 52% inhibition, minor poly(A) polymerase | Bacillus subtilis | |
2.7.7.19 | additional information | not inhibitory: rifampicin, streptolydigin, phosphate at 0.5 mM, cordycepin at 0.1 mM | Bacillus subtilis | |
2.7.7.19 | PO43- | not inhibitory | Bacillus subtilis | |
2.7.7.19 | rifampicin | not inhibitory | Bacillus subtilis | |
2.7.7.19 | UTP | - |
Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.19 | 0.143 | - |
ATP | major poly(A) polymerase, 37°C, pH 8.0 | Bacillus subtilis | |
2.7.7.19 | 0.4 | - |
ATP | major poly(A) polymerase, 37°C, pH 8.0 | Bacillus subtilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.7.19 | soluble | - |
Bacillus subtilis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.19 | Mg2+ | more active in presence of Mn2+ than Mg2+ | Bacillus subtilis | |
2.7.7.19 | Mn2+ | more active in presence of Mn2+ than Mg2+ | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.19 | 37000 | - |
gel filtration, minor poly(A) polymerase | Bacillus subtilis |
2.7.7.19 | 86000 | - |
gel filtration, major poly(A) polymerase | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.19 | Bacillus subtilis | - |
two isoforms | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.19 | ATP + RNA | primer required | Bacillus subtilis | diphosphate + RNA(A)n | - |
? | |
2.7.7.19 | ATP + RNA | enzyme also has cleavage activity | Bacillus subtilis | diphosphate + RNA(A)n | - |
? | |
2.7.7.19 | ATP + RNA | primer: poly(A) | Bacillus subtilis | diphosphate + RNA(A)n | - |
? | |
2.7.7.19 | ATP + RNA | primer: dephosphorylated poly(A), tRNA | Bacillus subtilis | diphosphate + RNA(A)n | - |
? |