EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.15 | - |
Rattus norvegicus |
EC Number | General Stability | Organism |
---|---|---|
2.7.7.15 | removal of detergents leads to rapid loss of activity and self-aggregation, high tendency to bind to glass and plastic surfaces even in the presence of detergents | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.15 | 4-chloromercuribenzoate | 80% inhibition at 0.0006 mM | Rattus norvegicus | |
2.7.7.15 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Rattus norvegicus | |
2.7.7.15 | N-ethylmaleimide | 80% inhibition at 1.5 mM; CTP and phosphocholine protect | Rattus norvegicus | |
2.7.7.15 | phosphate | maximal inhibition of 60% at 120 mM | Rattus norvegicus | |
2.7.7.15 | Sphingolipids | reversed by activating phospholipids | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.15 | 0.22 | - |
CTP | pH 7, 37°C | Rattus norvegicus | |
2.7.7.15 | 0.24 | - |
phosphocholine | pH 7, 37°C | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.7.15 | cytosol | - |
Rattus norvegicus | 5829 | - |
2.7.7.15 | membrane | - |
Rattus norvegicus | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.15 | 44500 | - |
2 * 44500, SDS-PAGE | Rattus norvegicus |
2.7.7.15 | 97000 | - |
gel filtration, glycerol density gradient centrifugation | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.15 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.15 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.15 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.15 | 47.5 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.15 | -70°C, 50 mM Tris-HCl, 150 mM NaCl, 1.0 mM EDTA, 2.0 mM DTT, 0.025% NaN3, pH 7.4, 0.03% Triton X-100, 200 mM phosphate, stable for several months | Rattus norvegicus |
2.7.7.15 | 4°C, 20% loss of activity per day | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.15 | CTP + choline phosphate | - |
Rattus norvegicus | diphosphate + CDPcholine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.15 | dimer | 2 * 44500, SDS-PAGE | Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.15 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.15 | 7 | - |
- |
Rattus norvegicus |